ArticleJournal of leukocyte biology2013
Technical advance: autofluorescence-based sorting: rapid and nonperturbing isolation of ultrapure neutrophils to determine cytokine production.
Article in Journal of leukocyte biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Illuminating Immunity: A Systematic Review of Immune Cell Autofluorescence.Journal of biophotonics · 2025Pooled it
- Oxygen levels determine the ability of glucocorticoids to influence neutrophil survival in inflammatory environments.Journal of leukocyte biology · 2013Trial
- Simplified flow cytometric quantification of human neutrophil extracellular traps (NETs).Journal of inflammation (London, England) · 2026Article
- Rapid autofluorescence flow cytometric analysis of agonist-induced neutrophil and eosinophil polarization reveals novel insights into 5-oxo-ETE-mediated granulocyte activation.Journal of inflammation (London, England) · 2025Article
- Single-Cell Transcriptome Profiling Reveals Conserved IFNγ-IL8 Signaling-Induced Antibacterial Neutrophil States during Bacterial Infection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Quantitative proteomics reveals tissue-specific, infection-induced and species-specific neutrophil protein signatures.Scientific reports · 2024Article
- Immunological shifts during early-stage Parkinson's disease identified with DNA methylation data on longitudinally collected blood samples.NPJ Parkinson's disease · 2024Article
- A secreted sirtuin fromScience advances · 2023Article
- Analysis of Neutrophil Responses to Biological Exposures.Current protocols · 2023Article
- Targeting Neutrophil βBiomolecules · 2023Review
- Article
- Label-free macrophage phenotype classification using machine learning methods.Scientific reports · 2023Article
- Isolation methods determine human neutrophil responses after stimulation.Frontiers in immunology · 2023Article
- Differential roles for the oxygen sensing enzymes PHD1 and PHD3 in the regulation of neutrophil metabolism and function.Wellcome open research · 2023Article
- Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics.STAR protocols · 2022Article
- Using the Autofluorescence Finder on the Sony ID7000Frontiers in bioengineering and biotechnology · 2022Article
- Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows.Scientific reports · 2020Article
- Article
- Autofluorescence flow sorting of breast cancer cell metabolism.Journal of biophotonics · 2017Article
- Engineering macrophages to eat cancer: from "marker of self" CD47 and phagocytosis to differentiation.Journal of leukocyte biology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
The technical limitations of isolating neutrophils without contaminating leukocytes, while concurrently minimizing neutrophil activation, is a barrier to determining specific neutrophil functions. We aimed to assess the use of FACS for generating highly pure quiescent neutrophil populations in an antibody-free environment. Peripheral blood human granulocytes and murine bone marrow-derived neutrophils were isolated by discontinuous Percoll gradient and flow-sorted using FSC/SSC profiles and differences in autofluorescence. Postsort purity was assessed by morphological analysis and flow cytometry. Neutrophil activation was measured in unstimulated-unsorted and sorted cells and in response to fMLF, LTB4, and PAF by measuring shape change, CD62L, and CD11b expression; intracellular calcium flux; and chemotaxis. Cytokine production by human neutrophils was also determined. Postsort human neutrophil purity was 99.95% (sem=0.03; n=11; morphological analysis), and 99.68% were CD16(+ve) (sem=0.06; n=11), with similar results achieved for murine neutrophils. Flow sorting did not alter neutrophil activation or chemotaxis, relative to presorted cells, and no differences in response to agonists were observed. Stimulated neutrophils produced IL-1β, although to a lesser degree than CXCL8/IL-8. The exploitation of the difference in autofluorescence between neutrophils and eosinophils by FACS is a quick and effective method for generating highly purified populations for subsequent in vitro study.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.