ArticleNucleic acids research2013
Caffeine suppresses homologous recombination through interference with RAD51-mediated joint molecule formation.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Caffeine inhibits nonhomologous end joining by impairing ligase IV/XRCC4 function.Nucleic acids research · 2026Article
- C8orf33 dictates DNA double-strand break repair choice by modulating KAT8-mediated H4K16 acetylation.Cell death & disease · 2025Article
- Curcumin Induces Homologous Recombination Deficiency by BRCA2 Degradation in Breast Cancer and Normal Cells.Cancers · 2025Article
- Article
- Coffee consumption, cancer, and healthy aging: epidemiological evidence and underlying mechanisms.GeroScience · 2025Review
- Exploring caffeine as a disruptor of membrane integrity and genomic stability in Staphylococcus aureus: functional and in silico analysis.Archives of microbiology · 2025Article
- Effects of Caffeine, a DNA Damage Response Inhibitor, on Papillomavirus Genome Replication.Pathogens (Basel, Switzerland) · 2022Article
- Yap1-mediated Flr1 expression reveals crosstalk between oxidative stress signaling and caffeine resistance inFrontiers in microbiology · 2022Article
- Ecdysteroid kinase-like (EcKL) paralogs confer developmental tolerance to caffeine inCurrent research in insect science · 2022Article
- Pleiotropic Effects of Caffeine Leading to Chromosome Instability and Cytotoxicity in Eukaryotic Microorganisms.Journal of microbiology and biotechnology · 2021Review
- HSF2BP negatively regulates homologous recombination in DNA interstrand crosslink repair.Nucleic acids research · 2020Article
- Low dose ionizing radiation strongly stimulates insertional mutagenesis in a γH2AX dependent manner.PLoS genetics · 2020Article
- The Homologous Recombination Machinery Orchestrates Post-replication DNA Repair During Self-renewal of Mouse Embryonic Stem Cells.Scientific reports · 2017Article
- Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA.Nature communications · 2017Article
- Cellular Dynamics of Rad51 and Rad54 in Response to Postreplicative Stress and DNA Damage in HeLa Cells.Molecules and cells · 2017Article
- v-Src Causes Chromosome Bridges in a Caffeine-Sensitive Manner by Generating DNA Damage.International journal of molecular sciences · 2016Article
- Article
- Caffeine impairs resection during DNA break repair by reducing the levels of nucleases Sae2 and Dna2.Nucleic acids research · 2015Article
- Caffeine inhibits gene conversion by displacing Rad51 from ssDNA.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caffeine is a widely used inhibitor of the protein kinases that play a central role in the DNA damage response. We used chemical inhibitors and genetically deficient mouse embryonic stem cell lines to study the role of DNA damage response in stable integration of the transfected DNA and found that caffeine rapidly, efficiently and reversibly inhibited homologous integration of the transfected DNA as measured by several homologous recombination-mediated gene-targeting assays. Biochemical and structural biology experiments revealed that caffeine interfered with a pivotal step in homologous recombination, homologous joint molecule formation, through increasing interactions of the RAD51 nucleoprotein filament with non-homologous DNA. Our results suggest that recombination pathways dependent on extensive homology search are caffeine-sensitive and stress the importance of considering direct checkpoint-independent mechanisms in the interpretation of the effects of caffeine on DNA repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.