ArticleObesity (Silver Spring, Md.)2013
Involvement of cathepsins B and L in inflammation and cholesterol trafficking protein NPC2 secretion in macrophages.
Article in Obesity (Silver Spring, Md.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 45 citations in OpenAlex.
- Exploring the Potential of Calebin-A in Targeting Obesity-Related Genes and Pathways.Journal of cellular and molecular medicine · 2026Article
- Identification of serum protein biomarkers in individuals with Niemann-Pick disease, type C1.Research square · 2026Article
- Identification of serum protein biomarkers in individuals with Niemann-Pick disease, type C1.medRxiv : the preprint server for health sciences · 2026Article
- Causal association between cathepsins and asthma: a Mendelian randomization study.Scientific reports · 2025Article
- Cathepsin B Levels Correlate with the Severity of Canine Myositis.Biomolecules · 2025Article
- The multifaceted role of vitreous hyalocytes: Orchestrating inflammation, angiomodulation and erythrophagocytosis in proliferative diabetic retinopathy.Journal of neuroinflammation · 2024Article
- The many roles of cathepsins in restenosis.Heliyon · 2024Article
- Cathepsin detection to identify malignant cells during robotic pulmonary resection.Translational lung cancer research · 2023Article
- A Turn-On Fluorescent Amino Acid Sensor Reveals Chloroquine's Effect on Cellular Amino Acids via Inhibiting Cathepsin L.ACS central science · 2023Article
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- The Impact of Inflammatory Stimuli on Xylosyltransferase-I Regulation in Primary Human Dermal Fibroblasts.Biomedicines · 2022Article
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- Elevated CTSL Gene Expression Correlated with Proinflammatory Cytokines in Omental Adipose Tissue of Patients with Obesity.Diabetes, metabolic syndrome and obesity : targets and therapy · 2022Article
- Increase in plasma Niemann-Pick disease type C2 protein is associated with poor prognosis of sepsis.Scientific reports · 2021Article
- Depletion of NK Cells Improves Cognitive Function in the Alzheimer Disease Mouse Model.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Systemic Investigation of Promoter-wide Methylome and Genome Variations in Gout.International journal of molecular sciences · 2020Article
- Identification of Interleukin-8-Reducing Lead Compounds Based on SAR Studies on Dihydrochalcone-Related Compounds in Human Gingival Fibroblasts (HGF-1 cells) In Vitro.Molecules (Basel, Switzerland) · 2020Article
- Bioinformatic Analysis of the Wound Peptidome Reveals Potential Biomarkers and Antimicrobial Peptides.Frontiers in immunology · 2020Article
- Cathepsins in Bacteria-Macrophage Interaction: Defenders or Victims of Circumstance?Frontiers in cellular and infection microbiology · 2020Review
- Association between Lysosomal Dysfunction and Obesity-Related Pathology: A Key Knowledge to Prevent Metabolic Syndrome.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
objectiveObesity and its related chronic inflammation are the major risk factors for developing metabolic disturbances. The roles of cathepsin cysteine proteases have been tied to inflammation and atherosclerosis. Cathepsins are important functional links between inflammation, cholesterol metabolism, and atherosclerosis in obesity. NPC2, a lysosomal protein, plays an important role in cholesterol trafficking. The objective of this study was to examine the regulation of cathepsins and NPC2 in adipose tissue and macrophages in obesity and the effect of modifying cathepsin activity in cholesterol metabolism and trafficking in macrophages. DESIGN AND
methodsCathepsins and NPC2 mRNA expression and protein secretion were detected in obese adipose tissue as well as 3T3-L1 adipocytes and Raw 264.7 macrophages in response to inflammatory stimuli and cathepsin inhibitors.
resultsIt was found that high-fat diet feeding altered the mRNA and protein expression levels of cathepsins B and L (CtB and CtL) and NPC2 in adipose tissue in mice; the differential regulation of these proteins was observed between adipose depots. In vitro studies showed that TNF-α reduces intracellular protein levels of CtB, CtL, and NPC2, but increases their secretion in 3T3-L1 adipocytes. Likewise, LPS stimulated the secretion of CtB and NPC2 in Raw 264.7 macrophages. Using the inhibitors of cathepsin enzymatic activity, it was found that CtB and CtL regulate TNF-α production, the expression and secretion of NPC2 protein, and the mRNA levels of the genes involved in cholesterol trafficking in macrophages.
conclusionThese findings suggest that CtB and CtL have a significant involvement in mediating the inflammatory response, in cholesterol trafficking, and in regulating NPC2 secretion.
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