ArticleBiotechnology letters2013
Morphology and growth of mammalian cells in a liquid/liquid culture system supported with oxygenated perfluorodecalin.
Article in Biotechnology letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A Pre-Clinical Model of Synovitis Using Ex vivo Human Synovial Tissue with Preserved Function and Architecture.Journal of visualized experiments : JoVE · 2026Article
- Therapeutic potential of Indonesian plant extracts in combating malaria and protozoan neglected tropical disease.BMC complementary medicine and therapies · 2024Article
- Bio-interactive nanoarchitectonics with two-dimensional materials and environments.Science and technology of advanced materials · 2022Review
- Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers.Materials today. Bio · 2021Article
- Microcarriers for Upscaling Cultured Meat Production.Frontiers in nutrition · 2020Review
- Materials nanoarchitectonics at two-dimensional liquid interfaces.Beilstein journal of nanotechnology · 2019Review
- Expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs) using a two-phase liquid/liquid system.Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) · 2017Article
- A scaffold-free surface culture of B16F10 murine melanoma cells based on magnetic levitation.Cytotechnology · 2016Article
- Past, present, and future of microcarrier-based tissue engineering.Journal of orthopaedic translation · 2015Review
- Murine precision-cut liver slices (PCLS): a new tool for studying tumor microenvironments and cell signaling ex vivo.Cell communication and signaling : CCS · 2014Article
- Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds.Bioprocess and biosystems engineering · 2014Article
- Liquid perfluorodecalin application for in situ extraction and enhanced naphthoquinones production in Arnebia euchroma cell suspension cultures.Applied biochemistry and biotechnology · 2014Article
- Enhanced cell sheet engineering through combination of single cells and spheroids on liquid interface using perfluorocarbon.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adherent A431, BHK-21, and C2C12 cells were cultured on a flexible interface formed between two immiscible liquid phases: (i) hydrophobic perfluorodecalin (PFD) and (ii) aqueous culture medium (DMEM). BHK-21 cells formed multicellular aggregates characterized by irregular shapes. A431, as well as C2C12 cells, grew as tight multicellular sheets of 3-D cells. Enhanced mass transfer and facilitated access of the cells to the O2 dissolved in PFD/DMEM by approx. 250 % and thereby increased the density of BHK-21 cells. Thus the liquid/liquid system is a simple, ready-to-use, and fully scalable (independent of vessel shapes); consequently it is a method for 3-D cultures of adherent animal cells in which the growth of anchorage-dependent cells is not limited by confluence effect.
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