ArticleNature methods2013
High-throughput tetrad analysis.
Article in Nature methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly.Biomolecules · 2023Review
- A method for quantifying sporulation efficiency and isolating meiotic progeny in non-GMO strains of Saccharomyces cerevisiae.Yeast (Chichester, England) · 2022Article
- Chromosome-Wide Characterization of Intragenic Crossover in Shiitake Mushroom,Journal of fungi (Basel, Switzerland) · 2021Article
- Computational Inference Software for Tetrad Assembly from Randomly Arrayed Yeast Colonies.G3 (Bethesda, Md.) · 2019Article
- Rapid Phenotypic and Genotypic Diversification After Exposure to the Oral Host Niche inGenetics · 2018Article
- Natural Variation inG3 (Bethesda, Md.) · 2018Article
- Detection of genomic loci associated with chromosomal recombination using high-density linkage mapping in Setaria.Scientific reports · 2017Article
- Haplotyping a Non-meiotic Diploid Fungal Pathogen Using Induced Aneuploidies and SNP/CGH Microarray Analysis.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Centromere Locations in Brassica A and C Genomes Revealed Through Half-Tetrad Analysis.Genetics · 2016Article
- Dissecting meiotic recombination based on tetrad analysis by single-microspore sequencing in maize.Nature communications · 2015Article
- Allelic variation, aneuploidy, and nongenetic mechanisms suppress a monogenic trait in yeast.Genetics · 2015Article
- BEST: barcode enabled sequencing of tetrads.Journal of visualized experiments : JoVE · 2014Article
- High-resolution mapping of complex traits with a four-parent advanced intercross yeast population.Genetics · 2013Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Tetrad analysis has been a gold-standard genetic technique for several decades. Unfortunately, the need to manually isolate, disrupt and space tetrads has relegated its application to small-scale studies and limited its integration with high-throughput DNA sequencing technologies. We have developed a rapid, high-throughput method, called barcode-enabled sequencing of tetrads (BEST), that uses (i) a meiosis-specific GFP fusion protein to isolate tetrads by FACS and (ii) molecular barcodes that are read during genotyping to identify spores derived from the same tetrad. Maintaining tetrad information allows accurate inference of missing genetic markers and full genotypes of missing (and presumably nonviable) individuals. An individual researcher was able to isolate over 3,000 yeast tetrads in 3 h, an output equivalent to that of almost 1 month of manual dissection. BEST is transferable to other microorganisms for which meiotic mapping is significantly more laborious.
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Registered trials
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