ArticleApplied and environmental microbiology2013
ProP is required for the survival of desiccated Salmonella enterica serovar typhimurium cells on a stainless steel surface.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 99 citations in OpenAlex.
- How long do bacteria, fungi, protozoa, and viruses retain their replication capacity on inanimate surfaces? A systematic review examining environmental resilience versus healthcare-associated infection risk by "fomite-borne risk assessment".Clinical microbiology reviews · 2024Pooled it
- Structure and function of Salmonella inner membrane.Archives of microbiology · 2026Review
- How Salmonella Works Under Osmotic and Desiccation Stresses.Comprehensive reviews in food science and food safety · 2026Review
- CommensalMicrobiology spectrum · 2025Article
- Stress Response Mechanisms ofFoods (Basel, Switzerland) · 2022Article
- Efficacy of Acidified Oils againstApplied and environmental microbiology · 2022Article
- Internalization of Salmonella in Leafy Greens and Impact on Acid Tolerance.Applied and environmental microbiology · 2022Article
- The Role of Egg Yolk in Modulating the Virulence ofFrontiers in cellular and infection microbiology · 2022Article
- Carvacrol and Thymol Combat Desiccation Resistance Mechanisms inMicroorganisms · 2021Article
- Alterations in the Transcriptional Landscape Allow Differential Desiccation Tolerance in Clinical Cronobacter sakazakii.Applied and environmental microbiology · 2021Article
- Microbial Air Quality in Healthcare Facilities.International journal of environmental research and public health · 2021Review
- Impact of the Resistance Responses to Stress Conditions Encountered in Food and Food Processing Environments on the Virulence and Growth Fitness of Non-TyphoidalFoods (Basel, Switzerland) · 2021Review
- Persistence of Pathogens on Inanimate Surfaces: A Narrative Review.Microorganisms · 2021Review
- Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress.BMC microbiology · 2020Article
- Identification of Novel Genes Mediating Survival ofFrontiers in microbiology · 2020Article
- Characterization of novel small RNAs (sRNAs) contributing to the desiccation response ofRNA biology · 2019Article
- The Microbial Lipopeptide Paenibacterin Disrupts Desiccation Resistance in Salmonella enterica Serovars Tennessee and Eimsbuettel.Applied and environmental microbiology · 2019Article
- The role of compatible solutes in desiccation resistance of Acinetobacter baumannii.MicrobiologyOpen · 2019Article
- RNA Sequencing-Based Transcriptional Overview of Xerotolerance in Cronobacter sakazakii SP291.Applied and environmental microbiology · 2019Article
- Role of Proline in Pathogen and Host Interactions.Antioxidants & redox signaling · 2019Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Consumers trust commercial food production to be safe, and it is important to strive to improve food safety at every level. Several outbreaks of food-borne disease have been caused by Salmonella strains associated with dried food. Currently we do not know the mechanisms used by Salmonella enterica serovar Typhimurium to survive in desiccated environments. The aim of this study was to discover the responses of S. Typhimurium ST4/74 at the transcriptional level to desiccation on a stainless steel surface and to subsequent rehydration. Bacterial cells were dried onto the same steel surfaces used during the production of dry foods, and RNA was recovered for transcriptomic analysis. Subsequently, dried cells were rehydrated and were again used for transcriptomic analysis. A total of 266 genes were differentially expressed under desiccation stress compared with a static broth culture. The osmoprotectant transporters proP, proU, and osmU (STM1491 to STM1494) were highly upregulated by drying. Deletion of any one of these transport systems resulted in a reduction in the long-term viability of S. Typhimurium on a stainless steel food contact surface. The proP gene was critical for survival; proP deletion mutants could not survive desiccation for long periods and were undetectable after 4 weeks. Following rehydration, 138 genes were differentially expressed, with upregulation observed for genes such as proP, proU, and the phosphate transport genes (pstACS). In time, this knowledge should prove valuable for understanding the underlying mechanisms involved in pathogen survival and should lead to improved methods for control to ensure the safety of intermediate- and low-moisture foods.
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