ArticleApplied and environmental microbiology2013
Occurrence of the transferable copper resistance gene tcrB among fecal enterococci of U.S. feedlot cattle fed copper-supplemented diets.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 65 citations in OpenAlex.
- Effect of combined piperine and amoxicillin as an efflux pump inhibitor for metal-tolerant and antibiotic - resistant E. coli in broilers.Veterinary research communications · 2026Article
- Green and chemical synthesis of CuMicrobiology spectrum · 2026Article
- The fecal resistome of beef cattle from conventional grain-fed and grass-fed systems in the Western United States.BMC microbiology · 2025Article
- The Role of Water as a Reservoir for Antibiotic-Resistant Bacteria.Antibiotics (Basel, Switzerland) · 2025Review
- Organic fertilization co-selects genetically linked antibiotic and metal(loid) resistance genes in global soil microbiome.Nature communications · 2024Article
- Unraveling the Role of Metals and Organic Acids in Bacterial Antimicrobial Resistance in the Food Chain.Antibiotics (Basel, Switzerland) · 2023Review
- Comparative Genomic Analysis of Enterococci across Sectors of the One Health Continuum.Microorganisms · 2023Article
- Factors associated with antimicrobial resistant enterococci in Canadian beef cattle: A scoping review.Frontiers in veterinary science · 2023Article
- Effect of high-copper diet on transference ofFrontiers in veterinary science · 2023Article
- Dynamic distribution and potential transmission of antibiotic resistance genes in activated sludge.Applied microbiology and biotechnology · 2022Article
- Experimental Evolution of Copper Resistance inAntibiotics (Basel, Switzerland) · 2022Article
- Effect of Protracted Free-Choice Chlortetracycline-Medicated Mineral for Anaplasmosis Control onMicroorganisms · 2021Article
- Heavy Metal Resistance inFrontiers in microbiology · 2021Article
- Effects of copper and zinc sources and inclusion levels of copper on weanling pig performance and intestinal microbiota.Journal of animal science · 2020Article
- Efflux proteins MacAB confer resistance to arsenite and penicillin/macrolide-type antibiotics in Agrobacterium tumefaciens 5A.World journal of microbiology & biotechnology · 2019Article
- Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles ofFrontiers in microbiology · 2019Article
- Distribution of theGenes · 2018Article
- Antimicrobial Resistance inMicrobiology spectrum · 2018Review
- Whole genome sequencing reveals widespread distribution of typhoidal toxin genes and VirB/D4 plasmids in bovine-associated nontyphoidal Salmonella.Scientific reports · 2018Article
- Antimicrobial Stewardship in Veterinary Medicine.Microbiology spectrum · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copper, an essential micronutrient, is supplemented in the diet at elevated levels to reduce morbidity and mortality and to promote growth in feedlot cattle. Gut bacteria exposed to copper can acquire resistance, which among enterococci is conferred by a transferable copper resistance gene (tcrB) borne on a plasmid. The present study was undertaken to investigate whether the feeding of copper at levels sufficient to promote growth increases the prevalence of the tcrB gene among the fecal enterococci of feedlot cattle. The study was performed with 261 crossbred yearling heifers housed in 24 pens, with pens assigned randomly to a 2×2 factorial arrangement of treatments consisting of dietary copper and a commercial linseed meal-based energy protein supplement. A total of 22 isolates, each identified as Enterococcus faecium, were positive for tcrB with an overall prevalence of 3.8% (22/576). The prevalence was higher among the cattle fed diets supplemented with copper (6.9%) compared to normal copper levels (0.7%). The tcrB-positive isolates always contained both erm(B) and tet(M) genes. Median copper MICs for tcrB-positive and tcrB-negative enterococci were 22 and 4 mM, respectively. The transferability of the tcrB gene was demonstrated via a filter-mating assay. Multilocus variable number tandem repeat analysis revealed a genetically diverse population of enterococci. The finding of a strong association between the copper resistance gene and other antibiotic (tetracycline and tylosin) resistance determinants is significant because enterococci remain potential pathogens and have the propensity to transfer resistance genes to other bacteria in the gut.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.