ArticleNature structural & molecular biology2013
Turning catalytically inactive human Argonaute proteins into active slicer enzymes.
Article in Nature structural & molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 108 citations in OpenAlex.
- Engineered human Argonaute 2 for targeted C-to-U RNA base editing.Nature biotechnology · 2026Article
- Structural basis for RISC assembly of human Argonaute2.Molecular cell · 2026Article
- SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data.Acta pharmaceutica Sinica. B · 2026Article
- C-terminal tagging impairs AGO2 function.RNA biology · 2025Article
- Conformational Dynamics of hAgo2 Silencing: Decoding Functional Divergence across Human Argonaute Paralogs.Journal of chemical information and modeling · 2025Article
- Endogenous Ribonucleases: Therapeutic Targeting of the Transcriptome Through Oligonucleotide-Triggered RNA Inactivation.Biomolecules · 2025Review
- Importance of an N-terminal structural switch in the distinction between small RNA-bound and free ARGONAUTE.Nature structural & molecular biology · 2025Article
- The structural basis for RNA slicing by human Argonaute2.Cell reports · 2025Article
- Argonaute2 modulates megakaryocyte development and sex-specific control of platelet protein expression and reactivity.Scientific reports · 2025Article
- The structural basis for RNA slicing by human Argonaute2.bioRxiv : the preprint server for biology · 2024Article
- DNA Hypomethylation Underlies Epigenetic Swapping betweenEpigenomes · 2024Article
- Catalytic residues of microRNA Argonautes play a modest role in microRNA star strand destabilization in C. elegans.Nucleic acids research · 2024Article
- Identification of an active RNAi pathway inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The catalytic activity of microRNA Argonautes plays a modest role in microRNA star strand destabilization inbioRxiv : the preprint server for biology · 2024Article
- Genome-wide identification, characterization and expression analysis of AGO, DCL, and RDR families in Chenopodium quinoa.Scientific reports · 2023Article
- Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2.Nature communications · 2022Article
- NgAgo possesses guided DNA nicking activity.Nucleic acids research · 2021Article
- Argonaute 5 family proteins play crucial roles in the defence against Cymbidium mosaic virus and Odontoglossum ringspot virus in Phalaenopsis aphrodite subsp. formosana.Molecular plant pathology · 2021Article
- Argonaute proteins: structures and their endonuclease activity.Molecular biology reports · 2021Review
- Germline AGO2 mutations impair RNA interference and human neurological development.Nature communications · 2020Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Argonaute proteins interact with small RNAs that guide them to complementary target RNAs, thus leading to inhibition of gene expression. Some but not all Argonaute proteins are endonucleases and can cleave the complementary target RNA. Here, we have mutated inactive human Ago1 and Ago3 and generated catalytic Argonaute proteins. We find that two short sequence elements at the N terminus are important for activity. In addition, PIWI-domain mutations in Ago1 may misarrange the catalytic center. Our work helps in understanding of the structural requirements that make an Argonaute protein an active endonucleolytic enzyme.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.