ArticleDevelopmental biology2013
PI3K regulates branch initiation and extension of cultured mammary epithelia via Akt and Rac1 respectively.
Article in Developmental biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Planar cell polarity-directed cell crawling drives polarized epithelial morphogenesis.Current biology : CB · 2026Article
- Bioinformatics Analysis of Candidate Genes Related to Fat Deposition in Yaks at Different Energy Levels.Current issues in molecular biology · 2025Article
- Distinct Requirements for Adaptor Proteins NCK1 and NCK2 in Mammary Gland Development.Journal of mammary gland biology and neoplasia · 2023Article
- Childhood adiposity, serum metabolites and breast density in young women.Breast cancer research : BCR · 2022Article
- Rac1 promotes kidney collecting duct integrity by limiting actomyosin activity.The Journal of cell biology · 2021Article
- Aging-Associated Alterations in Mammary Epithelia and Stroma Revealed by Single-Cell RNA Sequencing.Cell reports · 2020Article
- PtdIns(3,4,5)PProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Novel Molecular and Phenotypic Insights into Congenital Lung Malformations.American journal of respiratory and critical care medicine · 2018Article
- Coordination of Receptor Tyrosine Kinase Signaling and Interfacial Tension Dynamics Drives Radial Intercalation and Tube Elongation.Developmental cell · 2018Article
- Oleic acid stimulates HC11 mammary epithelial cells proliferation and mammary gland development in peripubertal mice through activation of CD36-CaOncotarget · 2018Article
- Microfabricated tissues for investigating traction forces involved in cell migration and tissue morphogenesis.Cellular and molecular life sciences : CMLS · 2017Review
- Article
- Rac1 modulates mammalian lung branching morphogenesis in part through canonical Wnt signaling.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix.Journal of visualized experiments : JoVE · 2016Article
- Quantitative proteomic analyses of mammary organoids reveals distinct signatures after exposure to environmental chemicals.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Inhibition of Sprouty2 polarizes macrophages toward an M2 phenotype by stimulation with interferon γ and Porphyromonas gingivalis lipopolysaccharide.Immunity, inflammation and disease · 2016Article
- Pushing, pulling, and squeezing our way to understanding mechanotransduction.Methods (San Diego, Calif.) · 2016Review
- mTOR Directs Breast Morphogenesis through the PKC-alpha-Rac1 Signaling Axis.PLoS genetics · 2015Article
- Regulation of tissue morphodynamics: an important role for actomyosin contractility.Current opinion in genetics & development · 2015Review
- Ror2 regulates branching, differentiation, and actin-cytoskeletal dynamics within the mammary epithelium.The Journal of cell biology · 2015Article
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2 authors.
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Abstract
The tree-like architecture of the mammary gland is generated by branching morphogenesis, which is regulated by many signals from the microenvironment. Here we examined how signaling downstream of phosphoinositide 3-kinase (PI3K) regulates different steps of mammary branching using three-dimensional culture models of the mammary epithelial duct. We found that PI3K was required for both branch initiation and elongation. Activated Akt was enhanced at branch initiation sites where its negative regulator, PTEN, was blocked by signaling via Sprouty2 (SPRY2); inhibiting Akt prevented branch initiation. The pattern of SPRY2 expression, and thus of Akt activation and branch initiation, was controlled by mechanical signaling from endogenous cytoskeletal contractility. In contrast, activated GTP-bound Rac1 localized to the leading edge of nascent branches and was required for branch elongation. These data suggest that the PI3K network integrates mechanical and biochemical signaling to control branching morphogenesis of mammary epithelial cells.
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