Evidence map›Paper›PMID 23664146›Full record

ArticleMetabolism: clinical and experimental2013

Pharmacological concentrations of irisin increase cell proliferation without influencing markers of neurite outgrowth and synaptogenesis in mouse H19-7 hippocampal cell lines.

Hyun-Seuk Moon, Fadime Dincer, Christos S Mantzoros

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Metabolism: clinical and experimental, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04121637 (To Investigate the Effect of Aerobic Exercise on Neurophysiological Values and Functionality in Individuals With Multiple Sclerosis.), which is not on this map. Cited by 96 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
96citing papers in PubMed, 3 pooled it
9.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04121637 naunknown statusnot on this mapstarted 2019, after this paper: background citation

To Investigate the Effect of Aerobic Exercise on Neurophysiological Values and Functionality in Individuals With Multiple Sclerosis.

TypeinterventionalSponsorFirat UniversityRan2019 to 2020Enrolled22ConditionsMultiple Sclerosis, Relapsing-Remitting, FNDC5, Irisin Hormone, Aerobic ExerciseArmsAerobic Exercise, Frenkel coordination exercises
3 · Its place in the literature

Who cites it

96 citing papers in PubMed, 3 syntheses or guidelines pooled it, 198 citations in OpenAlex.

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  18. Irisin/PGC-1α/FNDC5 pathway in Parkinson's disease: truth under the throes.Naunyn-Schmiedeberg's archives of pharmacology · 2024
    Review
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36 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Hyun-Seuk MoonDivision of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. hmoon@bidmc.harvard.edu.
Fadime Dincer
Christos S Mantzoros
Beth Israel Deaconess Medical Center · USHarvard University · USVA Boston Healthcare System · US

Funding

The Role of Leptin in the Maintenance of a Reduced Body WeightR01DK079929 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI MANTZOROS, CHRISTOS S · 2008 to 2012
$1.8M
Leptin and Adipokine PhysiologyK24DK081913 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI MANTZOROS, CHRISTOS S · 2008 to 2020
$1.5M
Role of Leptin in the Neuroendocrine Response to FastingR56DK058785 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI MANTZOROS, CHRISTOS S · 2005 to 2005
$85k
Leptin Signaling in HumansI01CX000422 · VA · VA BOSTON HEALTH CARE SYSTEM · PI MANTZOROS, CHRISTOS S · 2011 to 2014
–
CSRD VA I01 CX000422NIDDK NIH HHS K24 DK081913NIDDK NIH HHS R01 DK079929NIDDK NIH HHS R56 DK058785PHS HHS 58785PHS HHS 79929PHS HHS 81913
6 · The paper itself

Abstract

aims/hypothesisIrisin is a novel, myocyte secreted, hormone that has been proposed to mediate the beneficial effects of exercise on metabolism. Irisin is expressed, at lower levels, in human brains and knock-down of the precursor of irisin, FNDC5, decreases neural differentiation of mouse embryonic stem cells. No previous studies have evaluated whether irisin may directly regulate hippocampal neurogenesis in mouse hippocampal neuronal (HN) cells.

methodsHippocampal neurogenesis and irisin signaling were studied in vitro using mouse H19-7 HN cell lines.

resultsWe observed that cell proliferation is regulated by irisin in a dose-dependent manner in mouse H19-7 HN cells. Specifically, physiological concentrations of irisin, 5 to 10nmol/L, had no effect on cell proliferation when compared to control. By contrast, pharmacological concentrations of irisin, 50 to 100nmol/L, increased cell proliferation when compared to control. Similar to these results regarding irisin's effects on cell proliferation, we also observed that only pharmacological concentrations of irisin increased STAT3, but not AMPK and/or ERK, activation. Finally, we observed that irisin did not activate either microtubule-associated protein 2, a specific neurite outgrowth marker, or Synapsin, a specific synaptogenesis marker in mouse H19-7 HN cells. CONCLUSIONS/INTERPRETATIONS: Our data suggest that irisin, in pharmacological concentrations, increases cell proliferation in mouse H19-7 HN cells via STAT3, but not AMPK and/or ERK, signaling pathways. By contrast, neither physiological nor pharmacological concentrations of irisin alter markers of hippocampal neurogenesis in mouse H19-7 HN cell lines.

Indexed as

AMP-Activated Protein Kinase KinasesAnimalsBlotting, WesternCell DifferentiationCell LineCell ProliferationFibronectinsHippocampusHumansMAP Kinase Signaling SystemMiceNeuritesNeurogenesisProtein KinasesRecombinant ProteinsSignal TransductionAMP-Activated Protein Kinase KinasesFibronectinsFNDC5 protein, humanProtein KinasesRecombinant ProteinsSTAT3 Transcription FactorAMP-activated protein kinaseAMPKERKExtracellular signal-regulated kinaseHippocampal neurogenesisHippocampal neuronal cellsHNIrisinMAP2Microtubule-associated protein 2SignalingSignal transducer and activator of transcription 3STAT3

Identifiers

PMID23664146
PMCPMC4370428
OpenAlexW2069972139

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.