ArticleBiophysical journal2013
Single-cell force spectroscopy of probiotic bacteria.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 166 citations in OpenAlex.
- A detailed guideline for the fabrication of single bacterial probes used for atomic force spectroscopy.The European physical journal. E, Soft matter · 2015Guideline
- Material matters: a framework for integrating surface properties into built environment microbiome research.Applied and environmental microbiology · 2026Review
- Single-cell analysis of lipopolysaccharide-mediated heterogeneity in Escherichia coli adhesion and mechanics.Archives of microbiology · 2025Article
- Article
- Assessment of the biofilm formation capacities of Staphylococcus aureus strains Newman and Newman D2C in vitro and in vivo.Scientific reports · 2025Article
- Single-Cell Force Spectroscopy Uncovers Root Zone- and Bacteria-Specific Interactions.Angewandte Chemie (International ed. in English) · 2025Article
- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Serotype switching in Pseudomonas aeruginosa ST111 enhances adhesion and virulence.PLoS pathogens · 2024Article
- Nanoscale clustering of mycobacterial ligands and DC-SIGN host receptors are key determinants for pathogen recognition.Science advances · 2023Article
- In-Silico Analysis Highlights the Existence in Members ofMicroorganisms · 2023Article
- Hydroxyapatite Pellets as Versatile Model Surfaces for Systematic Adhesion Studies on Enamel: A Force Spectroscopy Case Study.ACS biomaterials science & engineering · 2022Article
- Anisotropic presentation of ligands on cargos modulates degradative function of phagosomes.Biophysical reports · 2022Article
- Quantifying molecular- to cellular-level forces in living cells.Journal of physics D: Applied physics · 2021Article
- The biophysics of bacterial infections: Adhesion events in the light of force spectroscopy.Cell surface (Amsterdam, Netherlands) · 2021Article
- Using Knock-Out Mutants to Investigate the Adhesion ofInternational journal of molecular sciences · 2021Article
- Quality control of direct cell-mineral adhesion measurements in air and liquid using inverse AFM imaging.RSC advances · 2021Article
- Plasmonic probing of the adhesion strength of single microbial cells.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Article
- Atomic Force Microscopy (AFM) As a Surface Mapping Tool in Microorganisms Resistant Toward Antimicrobials: A Mini-Review.Frontiers in pharmacology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell force spectroscopy is a powerful atomic force microscopy modality in which a single living cell is attached to the atomic force microscopy cantilever to quantify the forces that drive cell-cell and cell-substrate interactions. Although various single-cell force spectroscopy protocols are well established for animal cells, application of the method to individual bacterial cells remains challenging, mainly owing to the lack of appropriate methods for the controlled attachment of single live cells on cantilevers. We present a nondestructive protocol for single-bacterial cell force spectroscopy, which combines the use of colloidal probe cantilevers and of a bioinspired polydopamine wet adhesive. Living cells from the probiotic species Lactobacillus plantarum are picked up with a polydopamine-coated colloidal probe, enabling us to quantify the adhesion forces between single bacteria and biotic (lectin monolayer) or abiotic (hydrophobic monolayer) surfaces. These minimally invasive single-cell experiments provide novel, to our knowledge, insight into the specific and nonspecific forces driving the adhesion of L. plantarum, and represent a generic platform for studying the molecular mechanisms of cell adhesion in probiotic and pathogenic bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.