ArticleBiophysical journal2013
Quantitative analysis of self-association and mobility of annexin A4 at the plasma membrane.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells.FEBS open bio · 2026Article
- Expression of Annexin A4 in Cancer: A Tissue Microarray Study Involving More than 17,600 Cancers from 148 Tumor Entities.Cancers · 2026Article
- A conserved annexin A6-mediated membrane repair mechanism in muscle, heart, and nerve.JCI insight · 2022Article
- Article
- Annexins and Membrane Repair Dysfunctions in Muscular Dystrophies.International journal of molecular sciences · 2021Review
- Annexin Animal Models-From Fundamental Principles to Translational Research.International journal of molecular sciences · 2021Review
- Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.Aging · 2021Article
- Probing Membrane Protein Association Using Concentration-Dependent Number and Brightness.Angewandte Chemie (International ed. in English) · 2021Article
- Annexin A4 Is Dispensable for Hair Cell Development and Function.Frontiers in cell and developmental biology · 2021Article
- Protein Diffusion on Charged Biopolymers: DNA versus Microtubule.Biophysical journal · 2020Article
- Structure of the ALS Mutation Target Annexin A11 Reveals a Stabilising N-Terminal Segment.Biomolecules · 2020Article
- Using enhanced number and brightness to measure protein oligomerization dynamics in live cells.Nature protocols · 2019Article
- Self-association and subcellular localization of Puumala hantavirus envelope proteins.Scientific reports · 2019Article
- Annexins induce curvature on free-edge membranes displaying distinct morphologies.Scientific reports · 2018Article
- Fluorescence-based approaches for monitoring membrane receptor oligomerization.Journal of biosciences · 2018Review
- Influenza A matrix protein M1 multimerizes upon binding to lipid membranes.Biophysical journal · 2014Article
- Review
- Altered dynamics of a lipid raft associated protein in a kidney model of Fabry disease.Molecular genetics and metabolism · 2014Article
- Intracellular dynamics of archaeal FANCM homologue Hef in response to halted DNA replication.Nucleic acids research · 2013Article
- A toolbox of fluorescence microscopic approaches reveals dynamics and assembly of a membrane-associated protein.Biophysical journal · 2013Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a calcium-dependent manner. Annexin A4 (AnxA4) has been implicated in diverse cellular processes, including the regulation of exocytosis and ion-transport; however, its precise mechanistic role is not fully understood. AnxA4 has been shown to aggregate on lipid layers upon Ca(2+) binding in vitro, a characteristic that may be critical for its function. We have utilized advanced fluorescence microscopy to discern details on the mobility and self-assembly of AnxA4 after Ca(2+) influx at the plasma membrane in living cells. Total internal reflection microscopy in combination with Förster resonance energy transfer reveals that there is a delay between initial plasma membrane binding and the beginning of self-assembly and this process continues after the cytoplasmic pool has completely relocated. Number-and-brightness analysis suggests that the predominant membrane bound mobile form of the protein is trimeric. There also exists a pool of AnxA4 that forms highly immobile aggregates at the membrane. Fluorescence recovery after photobleaching suggests that the relative proportion of these two forms varies and is correlated with membrane morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.