ArticleBMC biotechnology2013
Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins.
Article in BMC biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- Recombinant production of spider silk protein in Physcomitrella photobioreactors.Plant cell reports · 2025Article
- Intracellular trafficking and glycosylation of hydroxyproline-O-glycosylation module in tobacco BY-2 cells is dependent on medium composition and transcriptome analysis.Scientific reports · 2023Article
- Optimising expression and extraction of recombinant proteins in plants.Frontiers in plant science · 2022Review
- Elastin-like polypeptide and γ-zein fusions significantly increase recombinant protein accumulation in soybean seeds.Transgenic research · 2021Article
- Antibodies induced by oral immunization of mice with a recombinant protein produced in tobacco plants harboringPlant cell, tissue and organ culture · 2021Article
- Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria.Transgenic research · 2020Article
- Characterization and Immunogenicity of HIV Envelope gp140 ZeraFrontiers in bioengineering and biotechnology · 2020Article
- Efficient Plant Production of Recombinant NS1 Protein for Diagnosis of Dengue.Frontiers in plant science · 2020Article
- Article
- Development of Systems for the Production of Plant-Derived Biopharmaceuticals.Plants (Basel, Switzerland) · 2019Review
- Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability.Biotechnology for biofuels · 2019Article
- Russell-Like Bodies in Plant Seeds Share Common Features With Prolamin Bodies and Occur Upon Recombinant Protein Production.Frontiers in plant science · 2019Article
- Hydrophobins: multifunctional biosurfactants for interface engineering.Journal of biological engineering · 2019Review
- Specific region affects the difference in accumulation levels between apple food allergen Mal d 1 and birch pollen allergen Bet v 1 which are expressed in vegetative tissues of transgenic rice.Plant molecular biology · 2018Article
- In silico and in vivo analyses of the mutated human tissue plasminogen activator (mtPA) and the antithetical effects of P19 silencing suppressor on its expression in two Nicotiana species.Scientific reports · 2018Article
- In-solution antibody harvesting with a plant-produced hydrophobin-Protein A fusion.Plant biotechnology journal · 2018Article
- Protein bodies: how the ER deals with high accumulation of recombinant proteins.Plant biotechnology journal · 2017Article
- Accumulation of foreign polypeptides to rice seed protein body type I using prolamin portion sequences.Plant cell reports · 2017Article
- Rapid generation of hypomorphic mutations.Nature communications · 2017Article
- High CO2 concentration as an inductor agent to drive production of recombinant phytotoxic antimicrobial peptides in plant biofactories.Plant molecular biology · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlants are recognized as an efficient and inexpensive system to produce valuable recombinant proteins. Two different strategies have been commonly used for the expression of recombinant proteins in plants: transient expression mediated by Agrobacterium; or stable transformation of the plant genome. However, the use of plants as bioreactors still faces two main limitations: low accumulation levels of some recombinant proteins and lack of efficient purification methods. Elastin-like polypeptide (ELP), hydrophobin I (HFBI) and Zera® are three fusion partners found to increase the accumulation levels of recombinant proteins and induce the formation of protein bodies (PBs) in leaves when targeted to the endoplasmic reticulum (ER) in transient expression assays. In this study the effects of ELP and HFBI fusion tags on recombinant protein accumulation levels and PB formation was examined in stable transgenic Nicotiana tabacum.
resultsThe accumulation of recombinant protein and PB formation was evaluated in two cultivars of Nicotiana tabacum transformed with green fluorescent protein (GFP) fused to ELP or HFBI, both targeted and retrieved to the ER. The ELP and HFBI tags increased the accumulation of the recombinant protein and induced the formation of PBs in leaves of stable transgenic plants from both cultivars. Furthermore, these tags induced the formation of PBs in a concentration-dependent manner, where a specific level of recombinant protein accumulation was required for PBs to appear. Moreover, agro-infiltration of plants accumulating low levels of recombinant protein with p19, a suppressor of post-transcriptional gene silencing (PTGS), increased accumulation levels in four independent transgenic lines, suggesting that PTGS might have caused the low accumulation levels in these plants.
conclusionThe use of ELP and HFBI tags as fusion partners in stable transgenic plants of tobacco is feasible and promising. In a constitutive environment, these tags increase the accumulation levels of the recombinant protein and induce the formation of PBs regardless of the cultivar used. However, a specific level of recombinant protein accumulation needs to be reached for PBs to form.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.