ArticleVox sanguinis2013
Proteomic analysis of the supernatant of red blood cell units: the effects of storage and leucoreduction.
Article in Vox sanguinis, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Red blood cell membrane proteome as a reporter of disease severity, transfusion impact and genetic background in transfusion-dependent β-thalassaemia.British journal of haematology · 2026Article
- Changes in hemoglobin oxidation and band 3 during blood storage impact oxygen sensing and mitochondrial bioenergetic pathways in the human pulmonary arterial endothelial cell model.Frontiers in physiology · 2023Article
- Red cell proteasome modulation by storage, redox metabolism and transfusion.Blood transfusion = Trasfusione del sangue · 2022Article
- Red Blood Cell Transfusion and Postoperative Delirium in Hip Fracture Surgery Patients: A Retrospective Observational Cohort Study.Anesthesiology research and practice · 2021Article
- Protect, repair, destroy or sacrifice: a role of oxidative stress biology in inter-donor variability of blood storage?Blood transfusion = Trasfusione del sangue · 2019Review
- Red blood cell storage lesion: causes and potential clinical consequences.Blood transfusion = Trasfusione del sangue · 2019Review
- A comparison of different methods of red blood cell leukoreduction and additive solutions on the accumulation of neutrophil-priming activity during storage.Transfusion · 2018Article
- Trauma-Induced Damage-Associated Molecular Patterns-Mediated Remote Organ Injury and Immunosuppression in the Acutely Ill Patient.Frontiers in immunology · 2018Review
- Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation.Transfusion · 2017Article
- Correlation of pre-operative plasma protein concentrations in cardiac surgery patients with bleeding outcomes using a targeted quantitative proteomics approach.Proteomics. Clinical applications · 2017Observational
- The accumulation of lipids and proteins during red blood cell storage: the roles of leucoreduction and experimental filtration.Blood transfusion = Trasfusione del sangue · 2017Review
- Supernatant protein biomarkers of red blood cell storage hemolysis as determined through an absolute quantification proteomics technology.Transfusion · 2016Article
- Leucoreduction of blood components: clinical and molecular evidence.Blood transfusion = Trasfusione del sangue · 2016Article
- A Comparative Study of the Effect of Leukoreduction and Pre-storage Leukodepletion on Red Blood Cells during Storage.Frontiers in molecular biosciences · 2016Article
- α-Enolase Causes Proinflammatory Activation of Pulmonary Microvascular Endothelial Cells and Primes Neutrophils Through Plasmin Activation of Protease-Activated Receptor 2.Shock (Augusta, Ga.) · 2015Article
- Established and theoretical factors to consider in assessing the red cell storage lesion.Blood · 2015Article
- Metabolomics of AS-5 RBC supernatants following routine storage.Vox sanguinis · 2015Article
- Classic and alternative red blood cell storage strategies: seven years of "-omics" investigations.Blood transfusion = Trasfusione del sangue · 2015Review
- Article
- Blood-banking techniques for plateletpheresis in swine.Journal of the American Association for Laboratory Animal Science : JAALAS · 2014Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundRed blood cell (RBC) transfusion is a life-saving intervention for critically ill patients; however, it has been linked to increased morbidity and mortality. We hypothesize that a number of important proteins accumulate during routine storage of RBCs, which may explain some of the adverse effects seen in transfused patients. STUDY
designFive RBC units were drawn and divided (half prestorage leucoreduced (LR-RBC) and half left as an unmodified control (RBC). The supernatant was separated on days 1 and 42 of storage and proteomic analyses completed with in-gel tryptic digestion and nano-liquid chromatography tandem mass spectrometry.
resultsIn RBC supernatants, 401 proteins were identified: 203 increased with storage, 114 decreased, and 84 were unchanged. In LR-RBC supernatant, 231 proteins were identified: 84 increased with storage, 30 decreased, and 117 were unchanged. Prestorage leucoreduction removed many platelet- and leucocyte-derived structural proteins; however, a number of intracellular proteins accumulated including peroxiredoxins (Prdx) 6 and latexin. The increases were confirmed by immunoblotting, including the T-phosphorylation of Prdx-6, indicating that it may be functioning as an active phospholipase. Active matrix metalloproteinase-9 also increased with a coinciding decrease in the metalloproteinase inhibitor 1 and cystatin C.
conclusionWe conclude that a number of proteins increase with RBC storage, which is partially ameliorated with leucoreduction, and transfusion of stored RBCs may introduce mediators that result in adverse events in the transfused host.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.