Evidence map›Paper›PMID 23662917›Full record

ArticleJournal of periodontal research2014

Differentiation of human dental stem cells reveals a role for microRNA-218.

I Gay, A Cavender, D Peto, Z Sun, A Speer, H Cao, B A Amendt

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of periodontal research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed, 1 pooled it
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 1 synthesis or guideline pooled it, 88 citations in OpenAlex.

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  7. International journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

I GayDental School, The University of Texas Health Science Center at Houston, Houston, TX, USA.
A Cavender
D Peto
Z Sun
A Speer
H Cao
B A Amendt
Texas A&M Health Science Center · USThe University of Texas Health Science Center at Houston · USIn-Q-Tel · US

Funding

Molecular Mechanisms of PITX2 During Craniofacial DevelopmentR01DE013941 · NIDCR · UNIVERSITY OF TULSA · PI AMENDT, BRAD A · 2000 to 2013
$4.2M
Mechanisms of FoxJ1 Tooth MorphogenesisR01DE018885 · NIDCR · UNIVERSITY OF IOWA · PI AMENDT, BRAD A · 2009 to 2012
$1.5M
NIDCR NIH HHS R01 DE013941NIDCR NIH HHS R01 DE018885
6 · The paper itself

Abstract

backgroundRegeneration of lost periodontium is the ultimate goal of periodontal therapy. Advances in tissue engineering have demonstrated the multilineage potential and plasticity of adult stem cells located in periodontal apparatus. However, it remains unclear how epigenetic mechanisms controlling signals determine tissue specification and cell lineage decisions. To date, no data are available on micro-RNA (miRNA) activity behind human-derived dental stem cells (DSCs). MATERIAL AND

methodsIn this study, we isolated periodontal ligament stem cells, dental pulp stem cells and gingival stem cells from extracted third molars; human bone marrow stem cells were used as a positive control. The expression of OCT4A and NANOG was confirmed in these undifferentiated cells. All cells were cultured under osteogenic inductive conditions and RUNX2 expression was analyzed as a marker of mineralized tissue differentiation. The miRNA expression profile was obtained at baseline and after osteogenic induction in all cell types.

resultsThe expression of RUNX2 demonstrated successful osteogenic induction of all cell types, which was confirmed by alizarin red stain. The analysis of 765 miRNAs demonstrated a shift in miRNA expression that occurred in all four stem cell types, including a decrease in hsa-mir-218 across all differentiated cell populations. Hsa-mir-218 targets RUNX2 and decreases RUNX2 expression in undifferentiated human DSCs. DSC mineralized tissue type differentiation is associated with a decrease in hsa-mir-218 expression.

conclusionThese data reveal a miRNA-regulated pathway for the differentiation of human DSCs and a select network of human miRNAs that control DSC osteogenic differentiation.

Indexed as

Adult Stem CellsAnthraquinonesCell Culture TechniquesCell DifferentiationCell SeparationColoring AgentsCore Binding Factor Alpha 1 SubunitDental PulpFlow CytometryGene TargetingGingivaHomeodomain ProteinsHumansImmunohistochemistryMesenchymal Stem CellsMicroRNAsalizarinAnthraquinonesColoring AgentsCore Binding Factor Alpha 1 SubunitHomeodomain ProteinsMicroRNAsMIRN218 microRNA, humanNanog Homeobox ProteinNANOG protein, humanOctamer Transcription Factor-3POU5F1 protein, humanRUNX2 protein, humancell differentiationmessenger RNAosteogenesisRNA induced silencing complexRUNX2stem cells

Identifiers

PMID23662917
PMCPMC3758777
OpenAlexW2000180714

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.