Evidence map›Paper›PMID 23662896›Full record

ArticleBioTechniques2013

Quantifying antibody binding on protein microarrays using microarray nonlinear calibration.

Xiaobo Yu, Garrick Wallstrom, Dewey Mitchell Magee, Ji Qiu, D Eliseo A Mendoza, Jie Wang, Xiaofang Bian, Morgan Graves, Joshua LaBaer

Abstract read
In one paragraph

Article in BioTechniques, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Article
  3. Parameter Reliability and Understanding Enzyme Function.Molecules (Basel, Switzerland) · 2022
    Review
  4. Article
  5. Article
  6. Protein Microarrays for Ocular Diseases.Methods in molecular biology (Clifton, N.J.) · 2021
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform.Journal of visualized experiments : JoVE · 2016
    Article
  12. Article
  13. Article
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xiaobo YuVirginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Garrick Wallstrom
Dewey Mitchell Magee
Ji Qiu
D Eliseo A Mendoza
Jie Wang
Xiaofang Bian
Morgan Graves
Joshua LaBaer

Funding

Biomarker Detection Using NAPPA Tumor Antigen ArraysU01CA117374 · NCI · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI ANDERSON, KAREN SUE, LABAER, JOSHUA · 2005 to 2015
$6.2M
Antibody Biomarkers for Early Detection of TuberculosisR01AI096213 · NIAID · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI MAGEE, D MITCHELL · 2011 to 2012
$1.0M
NCI NIH HHS 5U01CA117374NCI NIH HHS U01 CA117374NIAID NIH HHS R01 AI096213NIAID NIH HHS R01AI096213
6 · The paper itself

Abstract

We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A serological analysis of guinea pig Mycobacterium tuberculosis models showed that a larger number of putative antigen targets were identified with MiNC, which is consistent with the improved assay performance of protein microarrays. MiNC has the potential to be employed in biomedical research using multiplex antibody assays that need quantitation, including the discovery of antibody biomarkers, clinical diagnostics with multi-antibody signatures, and construction of immune mathematical models.

Indexed as

AlgorithmsAnimalsAntigens, BacterialBiomarkersCalibrationGuinea PigsHumansImmunoglobulin GMiceMycobacterium tuberculosisNonlinear DynamicsProtein Array AnalysisProtein BindingReference StandardsTuberculosis, PulmonaryTumor Suppressor Protein p53Antigens, BacterialBiomarkersImmunoglobulin GTumor Suppressor Protein p53

Identifiers

PMID23662896
PMCPMC4477631

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.