ArticleEnzyme research2013
Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii.
Article in Enzyme research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 10 citations in OpenAlex.
- Characterization of a Stable Form of Carboxypeptidase G2 (Glucarpidase), a Potential Biobetter Variant, From Acinetobacter sp. 263903-1.Molecular biotechnology · 2021Article
- Subtractive Proteomics and Immuno-informatics Approaches for Multi-peptide Vaccine Prediction AgainstInternational journal of peptide research and therapeutics · 2021Article
- Novel kinase platform for the validation of the anti-tubercular activities of Pelargonium sidoides (Geraniaceae).BMC biotechnology · 2020Article
- Synthesis of Phosphatidylserine and Its Stereoisomers: Their Role in Activation of Blood Coagulation.ACS medicinal chemistry letters · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acinetobacter baumannii is a multidrug resistant pathogenic bacteria associated with hospital acquired infections. This bacterium possesses a variety of resistance mechanisms which makes it more difficult to control the bacterium with conventional drugs, and, so far no effective drug treatment is available against it. Nucleoside diphosphate kinase is an important enzyme, which maintains the total nucleotide triphosphate pool inside the cell by the transfer of γ -phosphate from NTPs to NDPs. The role of nucleoside diphosphate kinase (Ndk) has also been observed in pathogenesis in other organisms. However, intensive studies are needed to decipher its other putative roles in Acinetobacter baumannii. In the present study, we have successfully cloned the gene encoding Ndk and achieved overexpression in bacterial host BL-21 (DE3). The overexpressed protein is further purified by nickel-nitrilotriacetic acid (Ni-NTA) chromatography.
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Registered trials
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