ArticleNature protocols2013
Measurement of phosphoinositides in the zebrafish Danio rerio.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- PI3P-dependent regulation of cell size and autophagy by phosphatidylinositol 5-phosphate 4-kinase.Life science alliance · 2023Article
- Voltage-sensing phosphatase (Vsp) regulates endocytosis-dependent nutrient absorption in chordate enterocytes.Communications biology · 2022Article
- Molecular basis of hUHRF1 allosteric activation for synergistic histone modification binding by PI5P.Science advances · 2022Article
- Quantitative Analysis of Polyphosphoinositide, Bis(monoacylglycero)phosphate, and Phosphatidylglycerol Species by Shotgun Lipidomics After Methylation.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Label-Free Quantification of Phosphoinositides in Drosophila by Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Simultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A novel mass assay to measure phosphatidylinositol-5-phosphate from cells and tissues.Bioscience reports · 2019Article
- Chemical fixation to arrest phospholipid signaling for chemical cytometry.Journal of chromatography. A · 2017Article
- The role of the PI(3,5)PMolecular and biochemical parasitology · 2017Article
- Fatty-acyl chain profiles of cellular phosphoinositides.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Article
- Importance of Radioactive Labelling to Elucidate Inositol Polyphosphate Signalling.Topics in current chemistry (Cham) · 2017Review
- The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output.Molecular cell · 2015Article
- The Lowe syndrome protein OCRL1 is required for endocytosis in the zebrafish pronephric tubule.PLoS genetics · 2015Article
- Article
- The cellular and physiological functions of the Lowe syndrome protein OCRL1.Traffic (Copenhagen, Denmark) · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Phosphoinositides represent a minor fraction of the total glycerolipids in cells. Despite the fact that phosphoinositides are present in small quantities, they have crucial roles during cell signaling and in regulating numerous intracellular processes. Measuring changes in the levels of different phosphoinositides in animals is difficult, but it is essential in order to define the important functions of specific members of the phosphoinositide family. Here we detail procedures for measuring phosphoinositides in 2-days-postfertilization (2-d.p.f.) embryos in zebrafish (Danio rerio). Both in vivo radiolabeling (using [(32)P]orthophosphate) followed by thin-layer or high-performance liquid chromatography (TLC or HPLC) analysis and specific in vitro phosphorylation assays (using [(32)P]γATP) permit the quantitative measurement of phosphoinositides. Normalization of both measurements can be achieved by the determination of total lipid phosphate in embryos. All the techniques described are relatively inexpensive and accessible to most laboratories with an interest in studying the effect of gene manipulation on phosphoinositide metabolism in zebrafish. All the procedures described herein will take up to 10 working days.
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Registered trials
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