ArticleMolecular endocrinology (Baltimore, Md.)2013
Research resource: small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes.
Article in Molecular endocrinology (Baltimore, Md.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
- Mapping ovarian cellular and molecular landscape across the lifespan of women: a scoping review.Human reproduction update · 2026Article
- MicroRNA Crossroads in PCOS and Endometriosis.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Review
- Bisphenol a Disrupts Steroidogenesis and Induces Apoptosis in Human Granulosa Cells Cultured In Vitro.International journal of molecular sciences · 2025Article
- Article
- High expression of miR-7974 predicts poor prognosis and is associated with autophagy in estrogen receptor-positive breast cancer.PloS one · 2025Article
- An integrated analysis of multiple datasets reveals novel gene signatures in human granulosa cells.Scientific data · 2024Article
- Single-cell RNA-seq analysis and cell-cluster deconvolution of the human preovulatory follicular fluid cells provide insights into the pathophysiology of ovarian hyporesponse.Frontiers in endocrinology · 2022Article
- Hsa-mir-548 family expression in human reproductive tissues.BMC genomic data · 2021Article
- Estrogen disorders: Interpreting the abnormal regulation of aromatase in granulosa cells (Review).International journal of molecular medicine · 2021Review
- Age-related miRNome landscape of cumulus oophorus cells during controlled ovarian stimulation protocols in IVF cycles.Human reproduction (Oxford, England) · 2021Article
- Big-Data Glycomics: Tools to Connect Glycan Biosynthesis to Extracellular Communication.Trends in biochemical sciences · 2021Review
- Long non-coding RNAs: novel players in the pathogenesis of polycystic ovary syndrome.Annals of translational medicine · 2021Review
- Cellular, Extracellular and Extracellular Vesicular miRNA Profiles of Pre-Ovulatory Follicles Indicate Signaling Disturbances in Polycystic Ovaries.International journal of molecular sciences · 2020Article
- Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri-implantation embryos.Biological reviews of the Cambridge Philosophical Society · 2019Review
- Differential miRNA Expression Profiles in Cumulus and Mural Granulosa Cells from Human Pre-ovulatory Follicles.MicroRNA (Shariqah, United Arab Emirates) · 2019Article
- Characterization of the miRNA regulators of the human ovulatory cascade.Scientific reports · 2018Article
- Molecular regulation of follicle-stimulating hormone synthesis, secretion and action.Journal of molecular endocrinology · 2018Review
- Differential long non-coding RNA expression profiles in human oocytes and cumulus cells.Scientific reports · 2018Article
- Hemoglobin enhances miRNA-144 expression and autophagic activation mediated inflammation of microglia via mTOR pathway.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signaling and are involved in the processes of folliculogenesis and oocyte maturation. Studies on gene expression and regulation in human granulosa cells are of interest due to their potential for estimating the oocyte viability and in vitro fertilization success. However, the posttranscriptional gene expression studies on micro-RNA (miRNA) level in the human ovary have been scarce. The current study determined the miRNA profile by deep sequencing of the 2 intrafollicular somatic cell types: mural and cumulus granulosa cells (MGCs and CGCs, respectively) isolated from women undergoing controlled ovarian stimulation and in vitro fertilization. Altogether, 936 annotated and 9 novel miRNAs were identified. Ninety of the annotated miRNAs were differentially expressed between MGCs and CGCs. Bioinformatic prediction revealed that TGFβ, ErbB signaling, and heparan sulfate biosynthesis were targeted by miRNAs in both granulosa cell populations, whereas extracellular matrix remodeling, Wnt, and neurotrophin signaling pathways were enriched among miRNA targets in MGCs. Two of the nine novel miRNAs found were of intronic origin: one from the aromatase and the other from the FSH receptor gene. The latter miRNA was predicted to target the activin signaling pathway. In addition to revealing the genome-wide miRNA signature in human granulosa cells, our results suggest that posttranscriptional regulation of gene expression by miRNAs could play an important role in the modification of gonadotropin signaling. miRNA expression studies could therefore lead to new prognostic markers in assisted reproductive technologies.
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