ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2013
Paneth cells expand from newly created and preexisting cells during repair after doxorubicin-induced damage.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Opposing Effects of LRIG1 and LRIG3 on EGFR Maintain Intestinal Homeostasis and Mitigate Likelihood of Colonic Neoplasia.Cellular and molecular gastroenterology and hepatology · 2026Article
- Goblet cell hyperplasia is not epithelial-autonomous in the Cftr knockout intestine.American journal of physiology. Gastrointestinal and liver physiology · 2022Article
- Paneth Cell Secretion in vivo Requires Expression of Tmem16a and Tmem16f.Gastro hep advances · 2022Article
- The phosphatase PRL-3 affects intestinal homeostasis by altering the crypt cell composition.Journal of molecular medicine (Berlin, Germany) · 2021Article
- PGD2 and CRTH2 counteract Type 2 cytokine-elicited intestinal epithelial responses during helminth infection.The Journal of experimental medicine · 2021Article
- Attenuation of Doxorubicin-Induced Small Intestinal Mucositis by Pectins is Dependent on Pectin's Methyl-Ester Number and Distribution.Molecular nutrition & food research · 2021Article
- Regulated IFN signalling preserves the stemness of intestinal stem cells by restricting differentiation into secretory-cell lineages.Nature cell biology · 2020Article
- Enteroendocrine Progenitor Cell-Enriched miR-7 Regulates Intestinal Epithelial Proliferation in an Xiap-Dependent Manner.Cellular and molecular gastroenterology and hepatology · 2020Article
- Article
- DeMISTifying Paneth Cell Maturation.Cellular and molecular gastroenterology and hepatology · 2019Article
- Cellular Plasticity of Defa4Cellular and molecular gastroenterology and hepatology · 2019Article
- Mist1 Expression Is Required for Paneth Cell Maturation.Cellular and molecular gastroenterology and hepatology · 2019Article
- Paneth Cells during Viral Infection and Pathogenesis.Viruses · 2018Review
- Cell Adhesion Molecule CD166/ALCAM Functions Within the Crypt to Orchestrate Murine Intestinal Stem Cell Homeostasis.Cellular and molecular gastroenterology and hepatology · 2017Article
- Depletion of enteric bacteria diminishes leukocyte infiltration following doxorubicin-induced small intestinal damage in mice.PloS one · 2017Article
- SHP-2 Phosphatase Prevents Colonic Inflammation by Controlling Secretory Cell Differentiation and Maintaining Host-Microbiota Homeostasis.Journal of cellular physiology · 2016Article
- Intestinal bacteria are necessary for doxorubicin-induced intestinal damage but not for doxorubicin-induced apoptosis.Gut microbes · 2016Article
- Defective goblet cell exocytosis contributes to murine cystic fibrosis-associated intestinal disease.The Journal of clinical investigation · 2015Article
- Peculiar composition of epithelial cells in follicle-associated intestinal crypts of Peyer's patches in the rat small intestine.The Journal of veterinary medical science · 2014Article
- Maternally acquired genotoxic Escherichia coli alters offspring's intestinal homeostasis.Gut microbesArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Paneth cell numbers increase following intestinal damage, but mechanisms driving this process are not understood. We hypothesized that the increase in Paneth cell numbers is due to recruitment of cells from a preexisting pool of secretory progenitors. Mice were given a single injection of doxorubicin (Dox), and intestinal tissue was collected 0-168 h after treatment. Paneth, goblet, and intermediate cells were counted and evaluated for cell morphology. Quantitative RT-PCR was used to measure expression of various genes associated with Paneth cell allocation and maturation. Paneth cells were birth dated using incorporation of thymidine analogs given before or after Dox. Staining revealed "intermediate" cells, which were rarely observed in control crypts but increased significantly in number 96 and 120 h after Dox treatment. Birth dating of intermediate cells 5 days after Dox treatment revealed that 24% of these cells took up thymidine analog given prior to Dox treatment and 36% took up thymidine analog given after Dox treatment. Quantitative RT-PCR demonstrated a significant increase in Spdef, Atoh1, Sox9, EphB3, Mist, Wnt5a, FGF-9, and FGF-18 mRNAs and a significant decrease in Indian hedgehog mRNA. Expansion of the Paneth cell compartment after Dox treatment is due to generation of new cells and recruitment of cells from an existing pool. These cells express Paneth and goblet biomarkers and are found only during repair. Expansion of these cells correlates temporally with reduced Indian hedgehog and increased FGF and Wnt mRNA. These findings are significant, as they provide a first step in understanding mechanisms of Paneth cell expansion during mucosal repair.
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