ArticleMolecular & cellular proteomics : MCP2013
Quantitative measurement of phosphoproteome response to osmotic stress in arabidopsis based on Library-Assisted eXtracted Ion Chromatogram (LAXIC).
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 71 citations in OpenAlex.
- Type One Protein Phosphatase 4aD Negatively Regulates Cotton (International journal of molecular sciences · 2025Article
- In Vivo Phosphorylation of the Cytosolic Glucose-6-Phosphate Dehydrogenase Isozyme G6PD6 in Phosphate-ResuppliedPlants (Basel, Switzerland) · 2023Article
- Genome-Wide Identification and Evolution of the GRF Gene Family and Functional Characterization ofInternational journal of molecular sciences · 2023Article
- G-Protein Phosphorylation: Aspects of Binding Specificity and Function in the Plant Kingdom.International journal of molecular sciences · 2022Review
- Proton and calcium pumping P-type ATPases and their regulation of plant responses to the environment.Plant physiology · 2021Review
- Plant Proteoforms Under Environmental Stress: Functional Proteins Arising From a Single Gene.Frontiers in plant science · 2021Review
- A tandem motif-based and structural approach can identify hidden functional phosphodiesterases.Computational and structural biotechnology journal · 2021Article
- Functional Crypto-Adenylate Cyclases Operate in Complex Plant Proteins.Frontiers in plant science · 2021Article
- Large-Scale Phosphoproteomic Study ofInternational journal of molecular sciences · 2020Article
- Unwinding BRAHMA Functions in Plants.Genes · 2020Review
- Signaling Toward Reactive Oxygen Species-Scavenging Enzymes in Plants.Frontiers in plant science · 2020Review
- Plasma Membrane-Associated Receptor-like Kinases Relocalize to Plasmodesmata in Response to Osmotic Stress.Plant physiology · 2019Article
- The Phosphoproteomic Response of Okra (International journal of molecular sciences · 2019Article
- Phosphorylation regulates the activity of INDETERMINATE-DOMAIN (IDD/BIRD) proteins in response to diverse environmental conditions.Plant signaling & behavior · 2019Review
- G protein subunit phosphorylation as a regulatory mechanism in heterotrimeric G protein signaling in mammals, yeast, and plants.The Biochemical journal · 2018Review
- Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome reveal growth regulators.Journal of experimental botany · 2018Article
- Three endoplasmic reticulum-associated fatty acyl-coenzyme a reductases were involved in the production of primary alcohols in hexaploid wheat (Triticum aestivum L.).BMC plant biology · 2018Article
- Degradation of the stress-responsive enzyme formate dehydrogenase by the RING-type E3 ligase Keep on Going and the ubiquitin 26S proteasome system.Plant molecular biology · 2018Article
- TheFrontiers in plant science · 2018Article
- Significant and unique changes in phosphorylation levels of four phosphoproteins in two apple rootstock genotypes under drought stress.Molecular genetics and genomics : MGG · 2017Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Global phosphorylation changes in plants in response to environmental stress have been relatively poorly characterized to date. Here we introduce a novel mass spectrometry-based label-free quantitation method that facilitates systematic profiling plant phosphoproteome changes with high efficiency and accuracy. This method employs synthetic peptide libraries tailored specifically as internal standards for complex phosphopeptide samples and accordingly, a local normalization algorithm, LAXIC, which calculates phosphopeptide abundance normalized locally with co-eluting library peptides. Normalization was achieved in a small time frame centered to each phosphopeptide to compensate for the diverse ion suppression effect across retention time. The label-free LAXIC method was further treated with a linear regression function to accurately measure phosphoproteome responses to osmotic stress in Arabidopsis. Among 2027 unique phosphopeptides identified and 1850 quantified phosphopeptides in Arabidopsis samples, 468 regulated phosphopeptides representing 497 phosphosites have shown significant changes. Several known and novel components in the abiotic stress pathway were identified, illustrating the capability of this method to identify critical signaling events among dynamic and complex phosphorylation. Further assessment of those regulated proteins may help shed light on phosphorylation response to osmotic stress in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.