ArticleAnalytical chemistry2013
An approach for separation and complete structural sequencing of heparin/heparan sulfate-like oligosaccharides.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Glycosaminoglycans and Their Role in Capillary Walls.Advances in experimental medicine and biology · 2026Review
- Simple One-Step Deuterated Peracetylation-MALDI-MS/MS (OSDPM) Strategy for Structural Sequencing of Glycosaminoglycan Oligosaccharides.Analytical chemistry · 2025Article
- Mass Spectrometry-Based Techniques to Elucidate the Sugar Code.Chemical reviews · 2022Review
- Straightforward Analysis of Sulfated Glycosaminoglycans by MALDI-TOF Mass Spectrometry from Biological Samples.Biology · 2022Article
- Developments in Mass Spectrometry for Glycosaminoglycan Analysis: A Review.Molecular & cellular proteomics : MCP · 2021Review
- Peracylation Coupled with Tandem Mass Spectrometry for Structural Sequencing of Sulfated Glycosaminoglycan Mixtures without Depolymerization.Journal of the American Society for Mass Spectrometry · 2020Article
- Structural Characterization of Glycosaminoglycan Carbohydrates Using Ultraviolet Photodissociation.Analytical chemistry · 2019Article
- Improved de novo sequencing of heparin/heparan sulfate oligosaccharides by propionylation of sites of sulfation.Carbohydrate research · 2018Article
- Negative Electron Transfer Dissociation Sequencing of 3-O-Sulfation-Containing Heparan Sulfate Oligosaccharides.Journal of the American Society for Mass Spectrometry · 2018Article
- Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.Journal of chromatography. A · 2018Article
- Structural elucidation of fucosylated chondroitin sulfates from sea cucumber using FTICR-MS/MS.European journal of mass spectrometry (Chichester, England) · 2018Article
- Glycosaminoglycanomics: where we are.Glycoconjugate journal · 2017Review
- A Multivariate Mixture Model to Estimate the Accuracy of Glycosaminoglycan Identifications Made by Tandem Mass Spectrometry (MS/MS) and Database Search.Molecular & cellular proteomics : MCP · 2017Article
- Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1.Journal of the American Chemical Society · 2016Article
- De Novo Sequencing of Complex Mixtures of Heparan Sulfate Oligosaccharides.Analytical chemistry · 2016Article
- Preparation and characterization of heparin hexasaccharide library with N-unsubstituted glucosamine residues.Glycoconjugate journal · 2015Article
- Heparan sulfate and heparin interactions with proteins.Journal of the Royal Society, Interface · 2015Review
- Profiling pneumococcal type 3-derived oligosaccharides by high resolution liquid chromatography-tandem mass spectrometry.Journal of chromatography. A · 2015Article
- GAG-ID: Heparan Sulfate (HS) and Heparin Glycosaminoglycan High-Throughput Identification Software.Molecular & cellular proteomics : MCP · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
As members of the glycosaminoglycan (GAG) family, heparin and heparan sulfate (HS) are responsible for mediation of a wide range of essential biological actions, most of which are mediated by specific patterns of modifications of regions of these polysaccharides. To fully understand the regulation of HS modification and the biological function of HS through its interactions with protein ligands, it is essential to know the specific HS sequences present. However, the sequencing of mixtures of HS oligosaccharides presents major challenges due to the lability of the sulfate modifications, as well as difficulties in separating isomeric HS chains. Here, we apply a sequential chemical derivatization strategy involving permethylation, desulfation, and trideuteroperacetylation to label original sulfation sites with stable and hydrophobic trideuteroacetyl groups. The derivatization chemistry differentiates between all possible heparin/HS sequences solely by glycosidic bond cleavages, without the need to generate cross-ring cleavages. This derivatization strategy combined with LC-MS/MS analysis has been used to separate and sequence five synthetic HS-like oligosaccharides of sizes up to dodecasaccharide, as well as a highly sulfated Arixtra-like heptamer. This strategy offers a unique capability for the sequencing of microgram quantities of HS oligosaccharide mixtures by LC-MS/MS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.