ArticleMolecular microbiology2013
Uncoupling of mRNA synthesis and degradation impairs adaptation to host temperature in Cryptococcus neoformans.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Puf4 is methylated and exhibits a temperature-dependent interactome inMicrobiology spectrum · 2026Article
- Human-Fungal Pathogen Interactions from the Perspective of Immunoproteomics Analyses.International journal of molecular sciences · 2024Review
- Cryptococcus neoformans requires the TVF1 gene for thermotolerance and virulence.Medical mycology · 2023Article
- Article
- Low Glucose Mediated Fluconazole Tolerance inJournal of fungi (Basel, Switzerland) · 2021Article
- Tools for Assessing Translation inJournal of fungi (Basel, Switzerland) · 2021Review
- Glucan Unmasking Identifies Regulators of Temperature-Induced Translatome Reprogramming in C. neoformans.mSphere · 2021Article
- The interplay of phenotype and genotype in Cryptococcus neoformans disease.Bioscience reports · 2020Review
- Article
- Thermotolerance in the pathogen Cryptococcus neoformans is linked to antigen masking via mRNA decay-dependent reprogramming.Nature communications · 2019Article
- Article
- Article
- Control of Candida albicans morphology and pathogenicity by post-transcriptional mechanisms.Cellular and molecular life sciences : CMLS · 2016Review
- Opposing PKA and Hog1 signals control the post-transcriptional response to glucose availability in Cryptococcus neoformans.Molecular microbiology · 2016Article
- Morphotype-specific effector functions of Cryptococcus neoformans PUM1.Scientific reports · 2016Article
- Puf4 regulates both splicing and decay of HXL1 mRNA encoding the unfolded protein response transcription factor in Cryptococcus neoformans.Eukaryotic cell · 2015Article
- Distinct and redundant roles of exonucleases in Cryptococcus neoformans: implications for virulence and mating.Fungal genetics and biology : FG & B · 2014Article
- Endoplasmic reticulum stress and fungal pathogenesis.Fungal biology reviews · 2014Article
- The ER stress response and host temperature adaptation in the human fungal pathogen Cryptococcus neoformans.Virulence · 2014Review
- The unfolded protein response (UPR) pathway in Cryptococcus.Virulence · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The pathogenic fungus Cryptococcus neoformans must overcome multiple stressors to cause disease in its human host. In this study, we report that C. neoformans rapidly and transiently repressed ribosomal protein (RP) transcripts during a transition from 30°C to host temperature. This repression was accompanied by accelerated mRNA degradation mediated by the major deadenylase, Ccr4, and influenced by the dissociable RNA polymerase II subunit, Rpb4. Destabilization and deadenylation of RP transcripts were impaired in an rpb4Δ mutant, suggesting that Rpb4 may be involved in host temperature-induced Ccr4-mediated decay. Accelerated decay of ER stress transcripts 1 h following a shift to host temperature was also impaired in the rpb4Δ mutant. In response to host temperature, Rpb4 moved from the nucleus to the cytoplasm, supporting a role for Rpb4 in coupling transcription and degradation. The PKH signalling pathway was implicated as a regulator of accelerated degradation of the RP transcripts, but not of the ER stress transcripts, revealing a further level of specificity. When transcription and degradation were uncoupled by deletion of Rpb4, growth at host temperature was impaired and virulence was attenuated. These data suggest that mRNA synthesis and decay are coupled in C. neoformans via Rpb4, and this tight coordination promotes host-temperature adaptation and pathogenicity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.