ArticlePloS one2013
Neurotoxicity induced by bupivacaine via T-type calcium channels in SH-SY5Y cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Calcium Nanoliposome Improves Glycemic Control in a Mouse Diabetes Mellitus Model.Pharmaceutical nanotechnology · 2026Article
- Mucoadhesive, antioxidant, and lubricant catechol-functionalized poly(phosphobetaine) as biomaterial nanotherapeutics for treating ocular dryness.Journal of nanobiotechnology · 2024Article
- Crocin alleviates neurotoxicity induced by bupivacaine in SH-SY5Y cells with inhibition of PI3K/AKT signaling.Genes & genomics · 2024Article
- Alpha lipoic acid attenuates evoked and spontaneous pain following surgical skin incision in rats.Channels (Austin, Tex.) · 2021Article
- Dexmedetomidine suppresses bupivacaine-induced parthanatos in human SH-SY5Y cells via the miR-7-5p/PARP1 axis-mediated ROS.Naunyn-Schmiedeberg's archives of pharmacology · 2021Article
- An appropriate ratio of unsaturated fatty acids is the constituent of hickory nut extract for neurite outgrowth in human SH-SY5Y cells.Food science & nutrition · 2020Article
- Knockdown of lincRNA PADNA promotes bupivacaine-induced neurotoxicity by miR-194/FBXW7 axis.Molecular medicine (Cambridge, Mass.) · 2020Article
- Bupivacaine-Induced Neurotoxicity Is Modulated by Epigenetic Axis of Long Noncoding RNA SNHG16 and Hsa-miR-132-3p.Neurotoxicity research · 2020Article
- Retraction: Neurotoxicity Induced by Bupivacaine via T-Type Calcium Channels in SH-SY5Y Cells.PloS one · 2020Article
- Inhibition of DNA Repair Protein Ku70 in High-Glucose Environment Aggravates the Neurotoxicity Induced by Bupivacaine in SH-SY5Y Cells.BioMed research international · 2020Article
- Ropivacaine mesylate exerts neurotoxicity via up-regulation of Fas/FasL expression in rat pheochromocytoma PC12 cells.American journal of translational research · 2019Article
- The Effects of Lidocaine with Epinephrine on Bupivacaine-Induced Cardiotoxicity.Turkish journal of anaesthesiology and reanimation · 2018Article
- Recent advances in the development of T-type calcium channel blockers for pain intervention.British journal of pharmacology · 2018Review
- CaMK II γ down regulation protects dorsal root ganglion neurons from ropivacaine hydrochloride neurotoxicity.Scientific reports · 2017Article
- Neurotoxicity Comparison of Two Types of Local Anaesthetics: Amide-Bupivacaine versus Ester-Procaine.Scientific reports · 2017Article
- In vivo Pharmacological Evaluations of Pilocarpine-Loaded Antioxidant-Functionalized Biodegradable Thermogels in Glaucomatous Rabbits.Scientific reports · 2017Article
- Local Anesthetic-Induced Neurotoxicity.International journal of molecular sciences · 2016Review
- Article
- Severe flaccid paraparesis following spinal anaesthesia: a sine materia occurrence.BMJ case reports · 2014Article
- T-type calcium channel blockers as neuroprotective agents.Pflugers Archiv : European journal of physiology · 2014Review
Corrections and comments
- Retraction · 2020-08-25Concerns/Issues about Image · Duplication of/in Image · Upgrade/Update of Prior Notice(s) ·
- Expression of concern · 2019-09-12Concerns/Issues about Image · Concerns/Issues about Third Party Involvement · Duplication of/in Image · Updated to Retraction ·
- Expression of concern
- Retracted
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is concern regarding neurotoxicity induced by the use of local anesthetics. A previous study showed that an overload of intracellular calcium is involved in the neurotoxic effect of some anesthetics. T-type calcium channels, which lower the threshold of action potentials, can regulate the influx of calcium ions. We hypothesized that T-type calcium channels are involved in bupivacaine-induced neurotoxicity. In this study, we first investigated the effects of different concentrations of bupivacaine on SH-SY5Y cell viability, and established a cell injury model with 1 mM bupivacaine. The cell viability of SH-SY5Y cells was measured following treatment with 1 mM bupivacaine and/or different dosages (10, 50, or 100 µM) of NNC 55-0396 dihydrochloride, an antagonist of T-type calcium channels for 24 h. In addition, we monitored the release of lactate dehydrogenase, cytosolic Ca(2+) ([Ca2+]i), cell apoptosis and caspase-3 expression. SH-SY5Y cells pretreated with different dosages (10, 50, or 100 µM) of NNC 55-0396 dihydrochloride improved cell viability, reduced lactate dehydrogenase release, inhibited apoptosis, and reduced caspase-3 expression following bupivacaine exposure. However, the protective effect of NNC 55-0396 dihydrochloride plateaued. Overall, our results suggest that T-type calcium channels may be involved in bupivacaine neurotoxicity. However, identification of the specific subtype of T calcium channels involved requires further investigation.
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