ArticlePloS one2013
Caspase-3 is involved in the signalling in erythroid differentiation by targeting late progenitors.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Induction of enucleation in primary and immortalized erythroid cells.International journal of hematology · 2022Review
- Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines.Communications biology · 2021Article
- Role of Extrinsic Apoptotic Signaling Pathway during Definitive Erythropoiesis in Normal Patients and in Patients with β-Thalassemia.International journal of molecular sciences · 2020Review
- Erythroid Differentiation and Heme Biosynthesis Are Dependent on a Shift in the Balance of Mitochondrial Fusion and Fission Dynamics.Frontiers in cell and developmental biology · 2020Article
- Disruption of erythroid nuclear opening and histone release in myelodysplastic syndromes.Cancer medicine · 2019Article
- MKK3 modulates JNK-dependent cell migration and invasion.Cell death & disease · 2019Article
- Non-apoptotic Caspase regulation of stem cell properties.Seminars in cell & developmental biology · 2018Review
- Modifications to a common phosphorylation network provide individualized control in caspases.The Journal of biological chemistry · 2018Article
- Trehalose Activates Autophagy and Prevents Hydrogen Peroxide-Induced Apoptosis in the Bone Marrow Stromal Cells.Iranian journal of pharmaceutical research : IJPR · 2018Article
- Evolution of caspase-mediated cell death and differentiation: twins separated at birth.Cell death and differentiation · 2017Review
- Non-apoptotic functions of caspases in myeloid cell differentiation.Cell death and differentiation · 2017Review
- Advances in understanding the mechanisms of erythropoiesis in homeostasis and disease.British journal of haematology · 2016Review
- Nuclear Condensation during Mouse Erythropoiesis Requires Caspase-3-Mediated Nuclear Opening.Developmental cell · 2016Article
- Eucommia ulmoides Oliv. (Du-Zhong) Lignans Inhibit Angiotensin II-Stimulated Proliferation by Affecting P21, P27, and Bax Expression in Rat Mesangial Cells.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Nerve growth factor-mediated inhibition of apoptosis post-caspase activation is due to removal of active caspase-3 in a lysosome-dependent manner.Cell death & disease · 2014Article
- Differential regulation of the apoptotic machinery during megakaryocyte differentiation and platelet production by inhibitor of apoptosis protein Livin.Cell death & disease · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A role for caspase activation in erythroid differentiation has been established, yet its precise mode of action remains elusive. A drawback of all previous investigations on caspase activation in ex vivo erythroid differentiation is the lack of an in vitro model producing full enucleation of erythroid cells. Using a culture system which renders nearly 100% enucleated red cells from human CD34(+) cells, we investigated the role of active caspase-3 in erythropoiesis. Profound effects of caspase-3 inhibition were found on erythroid cell growth and differentiation when inhibitors were added to CD34(+) cells at the start of the culture and showed dose-response to the concentration of inhibitor employed. Enucleation was only reduced as a function of the reduced maturity of the culture and the increased cell death of mature cells while the majority of cells retained their ability to extrude their nuclei. Cell cycle analysis after caspase-3 inhibition showed caspase-3 to play a critical role in cell proliferation and highlighted a novel function of this protease in erythroid differentiation, i.e. its contribution to cell cycle regulation at the mitotic phase. While the effect of caspase-3 inhibitor treatment on CD34(+) derived cells was not specific to the erythroid lineage, showing a similar reduction of cell expansion in myeloid cultures, the mechanism of action in both lineages appeared to be distinct with a strong induction of apoptosis causing the decreased yield of myeloid cells. Using a series of colony-forming assays we were able to pinpoint the stage at which cells were most sensitive to caspase-3 inhibition and found activated caspase-3 to play a signalling role in erythroid differentiation by targeting mature BFU-E and CFU-E but not early BFU-E.
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Registered trials
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