ArticlePloS one2013
Filtration parameters influencing circulating tumor cell enrichment from whole blood.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed.
- Gravity-based microfiltration reveals unexpected prevalence of circulating tumor cell clusters in ovarian and colorectal cancer.Communications medicine · 2025Article
- A FACS-based novel isolation technique identifies heterogeneous CTCs in oral squamous cell carcinoma.Frontiers in oncology · 2024Article
- Leukocyte Depletion and Size-Based Enrichment of Circulating Tumor Cells Using a Pressure-Sensing Microfiltration Device.ACS measurement science au · 2023Article
- Review
- Circulating tumour cells in the -omics era: how far are we from achieving the 'singularity'?British journal of cancer · 2022Review
- Negative enrichment of circulating tumor cells from unmanipulated whole blood with a 3D printed device.Scientific reports · 2021Article
- Exploration of Circulating Tumour Cell (CTC) Biology: A Paradigm Shift in Liquid Biopsy.Indian journal of clinical biochemistry : IJCB · 2021Review
- EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis.Clinical & experimental metastasis · 2021Article
- Circulating tumour cells as an indicator of early and systemic recurrence after surgical resection in pancreatic ductal adenocarcinoma.Scientific reports · 2021Article
- Circulating Tumor Cell Detection Technologies and Clinical Utility: Challenges and Opportunities.Cancers · 2020Review
- Clinical Significance of Circulating Tumor Cells in Gastrointestinal Carcinomas.Diagnostics (Basel, Switzerland) · 2020Review
- Microfluidics and Nanomaterial-based Technologies for Circulating Tumor Cell Isolation and Detection.Sensors (Basel, Switzerland) · 2020Review
- Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer.Sensors (Basel, Switzerland) · 2020Review
- Liquid biopsy and minimal residual disease - latest advances and implications for cure.Nature reviews. Clinical oncology · 2019Review
- A microfluidic device for label-free isolation of tumor cell clusters from unprocessed blood samples.Biomicrofluidics · 2019Article
- Biophysical Insights on the Enrichment of Cancer Cells from Whole Blood by (Affinity) Filtration.Scientific reports · 2019Article
- Self-Seeding Microwells to Isolate and Assess the Viability of Single Circulating Tumor Cells.International journal of molecular sciences · 2019Article
- EpCAMOncotarget · 2018Article
- Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells.Lab on a chip · 2018Article
- The Use of Microfluidic Technology for Cancer Applications and Liquid Biopsy.Micromachines · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filtration can achieve circulating tumor cell (CTC) enrichment from blood. Key parameters such as flow-rate, applied pressure, and fixation, vary largely between assays and their influence is not well understood. Here, we used a filtration system, to monitor these parameters and determine their relationships. Whole blood, or its components, with and without spiked tumor cells were filtered through track-etched filters. We characterize cells passing through filter pores by their apparent viscosity; the viscosity of a fluid that would pass with the same flow. We measured a ratio of 5·10(4)∶10(2)∶1 for the apparent viscosities of 15 µm diameter MDA-231 cells, 10 µm white cells and 90 fl red cells passing through a 5 µm pore. Fixation increases the pressure needed to pass cells through 8 µm pores 25-fold and halves the recovery of spiked tumor cells. Filtration should be performed on unfixed samples at a pressure of ∼10 mbar for a 1 cm(2) track-etched filter with 5 µm pores. At this pressure MDA-231 cells move through the filter in 1 hour. If fixation is needed for sample preservation, a gentle fixative should be selected. The difference in apparent viscosity between CTC and blood cells is key in optimizing recovery of CTC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.