ArticlePloS one2013
Porcine E. coli: virulence-associated genes, resistance genes and adhesion and probiotic activity tested by a new screening method.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Identification of Natural Mutations Responsible for Altered Infection Phenotypes of Salmonella enterica Clinical Isolates by Using Cell Line Infection Screens.Applied and environmental microbiology · 2021Article
- Proteomic Screening for Prediction and Design of Antimicrobial Peptides with AmpGram.International journal of molecular sciences · 2020Article
- Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates ofEXCLI journal · 2019Article
- Effect of different feed ingredients and additives on IPEC-J2 cells challenged with an enterotoxigenic Escherichia coli strain.Cytotechnology · 2016Article
- Genomic Microbial Epidemiology Is Needed to Comprehend the Global Problem of Antibiotic Resistance and to Improve Pathogen Diagnosis.Frontiers in microbiology · 2016Review
- Quantitative analysis of commensal Escherichia coli populations reveals host-specific enterotypes at the intra-species level.MicrobiologyOpen · 2015Article
- Prevalence of virulence determinants and antimicrobial resistance among commensal Escherichia coli derived from dairy and beef cattle.International journal of environmental research and public health · 2015Article
- Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins.Applied and environmental microbiology · 2013Article
- Genome Sequence of Porcine Escherichia coli Strain IMT8073, an Atypical Enteropathogenic E. coli Strain Isolated from a Piglet with Diarrhea.Genome announcements · 2013Article
- Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars.
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