ArticleFrontiers in cellular neuroscience2013
Confocal microscopy for astrocyte in vivo imaging: Recycle and reuse in microscopy.
Article in Frontiers in cellular neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 31 citations in OpenAlex.
- Intravital longitudinal cellular visualization of oral mucosa in a murine model based on rotatory side-view confocal endomicroscopy.Biomedical optics express · 2022Article
- Photonics tools begin to clarify astrocyte calcium transients.Neurophotonics · 2022Review
- Astrocyte-neuron signaling in the mesolimbic dopamine system: the hidden stars of dopamine signaling.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2021Review
- Optical clearing of living brains with MAGICAL to extendiScience · 2021Article
- AnBio-protocol · 2020Article
- Imaging the Neuroimmune Dynamics Across Space and Time.Frontiers in neuroscience · 2020Review
- A New Tool for In Vivo Study of Astrocyte Connexin 43 in Brain.Scientific reports · 2019Article
- Synapse-specific astrocyte gating of amygdala-related behavior.Nature neuroscience · 2017Article
- Retinal Macroglial Responses in Health and Disease.BioMed research international · 2016Review
- Reciprocal Regulation of Mitochondrial Dynamics and Calcium Signaling in Astrocyte Processes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Miniaturized fiber-coupled confocal fluorescence microscope with an electrowetting variable focus lens using no moving parts.Optics letters · 2015Article
- Structural and functional plasticity of astrocyte processes and dendritic spine interactions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Characterization of the BAC Id3-enhanced green fluorescent protein transgenic mouse line for in vivo imaging of astrocytes.Neurophotonics · 2014Article
- Imaging and monitoring astrocytes in health and disease.Frontiers in cellular neuroscience · 2014Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo imaging is one of the ultimate and fundamental approaches for the study of the brain. Two-photon laser scanning microscopy (2PLSM) constitutes the state-of-the-art technique in current neuroscience to address questions regarding brain cell structure, development and function, blood flow regulation and metabolism. This technique evolved from laser scanning confocal microscopy (LSCM), which impacted the field with a major improvement in image resolution of live tissues in the 1980s compared to widefield microscopy. While nowadays some of the unparalleled features of 2PLSM make it the tool of choice for brain studies in vivo, such as the possibility to image deep within a tissue, LSCM can still be useful in this matter. Here we discuss the validity and limitations of LSCM and provide a guide to perform high-resolution in vivo imaging of the brain of live rodents with minimal mechanical disruption employing LSCM. We describe the surgical procedure and experimental setup that allowed us to record intracellular calcium variations in astrocytes evoked by sensory stimulation, and to monitor intact neuronal dendritic spines and astrocytic processes as well as blood vessel dynamics. Therefore, in spite of certain limitations that need to be carefully considered, LSCM constitutes a useful, convenient, and affordable tool for brain studies in vivo.
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