ArticlePLoS genetics2013
Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Characterization of recombinase-based genetic parts and circuits using nanopore sequencing.Nature communications · 2026Article
- Large-scale mutational analysis uncovers molecular mechanisms governing dual RNA functions in transposons.Nucleic acids research · 2026Article
- Genomic and experimental characterization of unique Antarctic temperate phage Perkons reveals multiple integrases and complex lysogenic dynamics in Psychrobacillus sp. L4.BMC microbiology · 2026Article
- Deep-mutational scanning libraries using Tiled-Region Exchange mutagenesis.G3 (Bethesda, Md.) · 2026Article
- Temperature-Dependent Recombinase-Based Genetic Circuits.International journal of molecular sciences · 2025Article
- Site-specific DNA insertion into the human genome with engineered recombinases.Nature biotechnology · 2025Article
- ONE-STEP tagging: a versatile method for rapid site-specific integration by simultaneous reagent delivery.Nucleic acids research · 2025Article
- Structural basis of directionality control in large serine integrases.bioRxiv : the preprint server for biology · 2025Article
- Defining serine tRNA knockout as a strategy for effective repression of gene expression in organisms with a recoded genome.Nucleic acids research · 2025Article
- Next generation synthetic memory via intercepting recombinase function.Nature communications · 2023Article
- Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases.Nature biotechnology · 2023Article
- Phylogenomic analyses and host range prediction of cluster P mycobacteriophages.G3 (Bethesda, Md.) · 2022Article
- Model-guided engineering of DNA sequences with predictable site-specific recombination rates.Nature communications · 2022Article
- Construction of an IS-FreeFrontiers in bioengineering and biotechnology · 2021Article
- Oligo-Mediated Recombineering and its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases.Nature communications · 2019Article
- Identification of an Integrase That Responsible for Precise Integration and Excision ofFrontiers in microbiology · 2019Article
- Article
- Serine Integrase attP Binding and Specificity.Journal of molecular biology · 2018Article
- Diverse target gene modifications in Plasmodium falciparum using Bxb1 integrase and an intronic attB.Parasites & vectors · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements. These features make them attractive for genome engineering and synthetic genetics, although the basis for DNA site selection is poorly understood. Here we show that attP selection is determined through multiple proofreading steps that reject non-attP substrates, and that discrimination of attP and attB involves two critical site features: the outermost 5-6 base pairs of attP that are required for Int binding and recombination but antagonize attB function, and the "discriminators" at positions -15/+15 that determine attB identity but also antagonize attP function. Thus, although the attachment sites differ in length and sequence, only two base changes are needed to convert attP to attL, and just two more from attL to attB. The opposing effect of site identifiers ensures that site schizophrenia with dual identities does not occur.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.