ArticlePLoS pathogens2013
The production of monokaryotic hyphae by Cryptococcus neoformans can be induced by high temperature arrest of the cell cycle and is independent of same-sex mating.
Article in PLoS pathogens, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Ovoid cell is an inducible small-sized morphotype that enhances proliferation and antifungal drug tolerance in the human fungal pathogen Cryptococcus neoformans.PLoS pathogens · 2026Article
- Is Cryptococcus neoformans a pleomorphic fungus?Current opinion in microbiology · 2024Review
- Determination of Ploidy Levels and Nuclear DNA Content inJournal of fungi (Basel, Switzerland) · 2024Article
- Host-Pathogen Interactions and Correlated Factors That Are Affected in Replicative-AgedJournal of fungi (Basel, Switzerland) · 2024Article
- Dynamic genome plasticity during unisexual reproduction in the human fungal pathogen Cryptococcus deneoformans.PLoS genetics · 2021Article
- A cyclin protein governs the infectious and sexual life cycles of Cryptococcus neoformans.Science China. Life sciences · 2021Article
- On the History and Applications of Congenic Strains inPathogens (Basel, Switzerland) · 2020Review
- Article
- Pheromone independent unisexual development in Cryptococcus neoformans.PLoS genetics · 2017Article
- Unique roles of the unfolded protein response pathway in fungal development and differentiation.Scientific reports · 2016Article
- The lncRNA RZE1 Controls Cryptococcal Morphological Transition.PLoS genetics · 2015Article
- The morphotype heterogeneity in Cryptococcus neoformans.Current opinion in microbiology · 2015Review
- Morphology and its underlying genetic regulation impact the interaction between Cryptococcus neoformans and its hosts.Medical mycology · 2015Article
- Unisexual versus bisexual mating in Cryptococcus neoformans: Consequences and biological impacts.Fungal genetics and biology : FG & B · 2015Review
- Sexual reproduction of human fungal pathogens.Cold Spring Harbor perspectives in medicine · 2014Review
- Cryptococcus neoformans and Cryptococcus gattii, the etiologic agents of cryptococcosis.Cold Spring Harbor perspectives in medicine · 2014Review
- Unisexual reproduction of Cryptococcus gattii.PloS one · 2014Article
- Article
- Unisexual reproduction enhances fungal competitiveness by promoting habitat exploration via hyphal growth and sporulation.Eukaryotic cell · 2013Article
- Shaping up for battle: morphological control mechanisms in human fungal pathogens.PLoS pathogens · 2013Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Cryptococcus neoformans is a heterothallic fungal pathogen of humans and animals. Although the fungus grows primarily as a yeast, hyphae are produced during the sexual phase and during a process called monokaryotic fruiting, which is also believed to involve sexual reproduction, but between cells of the same mating type. Here we report a novel monokaryotic fruiting mechanism that is dependent on the cell cycle and occurs in haploid cells in the absence of sexual reproduction. Cells grown at 37°C were found to rapidly produce hyphae (∼4 hrs) and at high frequency (∼40% of the population) after inoculation onto hyphae-inducing agar. Microscopic examination of the 37°C seed culture revealed a mixture of normal-sized and enlarged cells. Micromanipulation of single cells demonstrated that only enlarged cells were able to produce hyphae and genetic analysis confirmed that hyphae did not arise from α-α mating or endoduplication. Cell cycle analysis revealed that cells grown at 37°C had an increased population of cells in G2 arrest, with the proportion correlated with the frequency of monokaryotic fruiting. Cell sorting experiments demonstrated that enlarged cells were only found in the G2-arrested population and only this population contained cells able to produce hyphae. Treatment of cells at low temperature with the G2 cell cycle arrest agent, nocodazole, induced hyphal growth, confirming the role of the cell cycle in this process. Taken together, these results reveal a mating-independent mechanism for monokaryotic fruiting, which is dependent on the cell cycle for induction of hyphal competency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.