ArticlePLoS computational biology2013
A kinetic platform to determine the fate of nitric oxide in Escherichia coli.
Article in PLoS computational biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Spatial transcriptome-guided multi-scale framework connects P. aeruginosa metabolic states to oxidative stress biofilm microenvironment.PLoS computational biology · 2024Article
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- Pseudomonas aeruginosa prioritizes detoxification of hydrogen peroxide over nitric oxide.BMC research notes · 2021Article
- Article
- Flavohaemoglobin: the pre-eminent nitric oxide-detoxifying machine of microorganisms.F1000Research · 2020Review
- Synergy Screening Identifies a Compound That Selectively Enhances the Antibacterial Activity of Nitric Oxide.Frontiers in bioengineering and biotechnology · 2020Article
- Quantitative Modeling Extends the Antibacterial Activity of Nitric Oxide.Frontiers in physiology · 2020Article
- Clustering as a Means To Control Nitrate Respiration Efficiency and Toxicity in Escherichia coli.mBio · 2019Article
- Iron-Sulfur Cluster Repair Contributes to Yersinia pseudotuberculosis Survival within Deep Tissues.Infection and immunity · 2019Article
- Transcriptional Regulation Contributes to Prioritized Detoxification of Hydrogen Peroxide over Nitric Oxide.Journal of bacteriology · 2019Article
- Design, Synthesis and Biological Evaluation of Nitrate Derivatives of Sauropunol A and B as Potent Vasodilatory Agents.Molecules (Basel, Switzerland) · 2019Article
- A platform of genetically engineered bacteria as vehicles for localized delivery of therapeutics: Toward applications for Crohn's disease.Bioengineering & translational medicine · 2018Article
- Development of Cell-Based Sentinels for Nitric Oxide: Ensuring Marker Expression and Unimodality.ACS synthetic biology · 2018Article
- Persister formation in Escherichia coli can be inhibited by treatment with nitric oxide.Free radical biology & medicine · 2016Article
- Discovery and dissection of metabolic oscillations in the microaerobic nitric oxide response network of Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Construction and Experimental Validation of a Quantitative Kinetic Model of Nitric Oxide Stress in EnterohemorrhagicBioengineering (Basel, Switzerland) · 2016Article
- Article
- A Kinetic Platform to Determine the Fate of Hydrogen Peroxide in Escherichia coli.PLoS computational biology · 2015Article
- Community behavior and spatial regulation within a bacterial microcolony in deep tissue sites serves to protect against host attack.Cell host & microbe · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitric oxide (NO•) is generated by the innate immune response to neutralize pathogens. NO• and its autoxidation products have an extensive biochemical reaction network that includes reactions with iron-sulfur clusters, DNA, and thiols. The fate of NO• inside a pathogen depends on a kinetic competition among its many targets, and is of critical importance to infection outcomes. Due to the complexity of the NO• biochemical network, where many intermediates are short-lived and at extremely low concentrations, several species can be measured, but stable products are non-unique, and damaged biomolecules are continually repaired or regenerated, kinetic models are required to understand and predict the outcome of NO• treatment. Here, we have constructed a comprehensive kinetic model that encompasses the broad reactivity of NO• in Escherichia coli. The incorporation of spontaneous and enzymatic reactions, as well as damage and repair of biomolecules, allowed for a detailed analysis of how NO• distributes in E. coli cultures. The model was informed with experimental measurements of NO• dynamics, and used to identify control parameters of the NO• distribution. Simulations predicted that NO• dioxygenase (Hmp) functions as a dominant NO• consumption pathway at O2 concentrations as low as 35 µM (microaerobic), and interestingly, loses utility as the NO• delivery rate increases. We confirmed these predictions experimentally by measuring NO• dynamics in wild-type and mutant cultures at different NO• delivery rates and O2 concentrations. These data suggest that the kinetics of NO• metabolism must be considered when assessing the importance of cellular components to NO• tolerance, and that models such as the one described here are necessary to rigorously investigate NO• stress in microbes. This model provides a platform to identify novel strategies to potentiate the effects of NO•, and will serve as a template from which analogous models can be generated for other organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.