ArticleJournal of virology2013
The resistance protein Tm-1 inhibits formation of a Tomato mosaic virus replication protein-host membrane protein complex.
Article in Journal of virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Tobamoviruses: Advances in Molecular Biology, Host Interactions and Integrated Disease Management.Biology · 2026Review
- Transcriptome-metabolome integration reveals MYB/WRKY-RGA network and phenylalanine metabolism in potato late blight resistance.BMC plant biology · 2026Article
- Rosemary essential oil: chemical composition, antiviral, cytotoxicity, and in silico molecular docking analysis against tobacco mosaic virus.Scientific reports · 2025Article
- Genomic Diversity of Tomato Brown Rugose Fruit Virus in Canadian Greenhouse Production Systems.Viruses · 2025Article
- Tobacco Mosaic Virus Movement: From Capsid Disassembly to Transport Through Plasmodesmata.Viruses · 2025Review
- Genome-wide analysis of tobacco NtTOM1/TOM3 gene family and identification of NtTOM1a/ NtTOM3a response to tobacco mosaic virus.BMC plant biology · 2024Article
- Plant Immunity against Tobamoviruses.Viruses · 2024Review
- Cell Fractionation and the Identification of Host Proteins Involved in Plant-Virus Interactions.Pathogens (Basel, Switzerland) · 2024Review
- Plant Protection against Viruses: An Integrated Review of Plant Immunity Agents.International journal of molecular sciences · 2023Review
- Understanding tobamovirus-plant interactions: implications for breeding resistance to tomato brown rugose fruit virus.Journal of plant pathology : an international journal of the Italian Phytopathological Society · 2023Review
- Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective.Plants (Basel, Switzerland) · 2022Review
- Host factors against plant viruses.Molecular plant pathology · 2019Review
- The Plant Noncanonical Antiviral Resistance Protein JAX1 Inhibits Potexviral Replication by Targeting the Viral RNA-Dependent RNA Polymerase.Journal of virology · 2019Article
- Binding studies between cytosinpeptidemycin and the superfamily 1 helicase protein of tobacco mosaic virus.RSC advances · 2018Article
- TomatoHorticulture research · 2018Article
- Plant immunity against viruses: antiviral immune receptors in focus.Annals of botany · 2017Review
- Immune Receptors and Co-receptors in Antiviral Innate Immunity in Plants.Frontiers in microbiology · 2016Review
- Structural basis for the recognition-evasion arms race between Tomato mosaic virus and the resistance gene Tm-1.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Crop immunity against viruses: outcomes and future challenges.Frontiers in plant science · 2014Review
- Crystallization and preliminary X-ray crystallographic analysis of the inhibitory domain of the tomato mosaic virus resistance protein Tm-1.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Tm-1 gene of tomato confers resistance to Tomato mosaic virus (ToMV). Tm-1 encodes a protein that binds ToMV replication proteins and inhibits the RNA-dependent RNA replication of ToMV. The replication proteins of resistance-breaking mutants of ToMV do not bind Tm-1, indicating that the binding is important for inhibition. In this study, we analyzed how Tm-1 inhibits ToMV RNA replication in a cell-free system using evacuolated tobacco protoplast extracts. In this system, ToMV RNA replication is catalyzed by replication proteins bound to membranes, and the RNA polymerase activity is unaffected by treatment with 0.5 M NaCl-containing buffer and remains associated with membranes. We show that in the presence of Tm-1, negative-strand RNA synthesis is inhibited; the replication proteins associate with membranes with binding that is sensitive to 0.5 M NaCl; the viral genomic RNA used as a translation template is not protected from nuclease digestion; and host membrane proteins TOM1, TOM2A, and ARL8 are not copurified with the membrane-bound 130K replication protein. Deletion of the polymerase read-through domain or of the 3' untranslated region (UTR) of the genome did not prevent the formation of complexes between the 130K protein and the host membrane proteins, the 0.5 M NaCl-resistant binding of the replication proteins to membranes, and the protection of the genomic RNA from nucleases. These results indicate that Tm-1 binds ToMV replication proteins to inhibit key events in replication complex formation on membranes that precede negative-strand RNA synthesis.
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