ArticleJournal of clinical microbiology2013
Targeting the treponemal microbiome of digital dermatitis infections by high-resolution phylogenetic analyses and comparison with fluorescent in situ hybridization.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-Analysis of Bovine Digital Dermatitis Microbiota Reveals Distinct Microbial Community Structures Associated With Lesions.Frontiers in cellular and infection microbiology · 2021Pooled it
- Detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2026Article
- Altered splenic T-cell subpopulation dynamics in a murine model of bovine digital dermatitis-associatedFrontiers in immunology · 2026Article
- Review
- Application of 16S rRNA gene amplicon sequencing in the investigation of novel ovine skin lesions in Norway.Frontiers in veterinary science · 2026Article
- Bacterial community analysis of treponeme-associated hoof disease in free-ranging elk (Applied and environmental microbiology · 2025Article
- Molecular Screening for Digital Dermatitis-Associated Treponemes in Bovine Ischaemic Teat Necrosis Lesions and Milk in Dairy Cattle.Pathogens (Basel, Switzerland) · 2024Article
- Correlation of lesion severity with bacterial changes in Treponeme-Associated Hoof Disease from free-roaming wild elk (Cervus canadensis).Animal microbiome · 2024Article
- Assessment of immunological response to digital dermatitis pathogen derived antigens following infection, recovery, and reinfection.Frontiers in veterinary science · 2024Article
- Development of a multiplex quantitative PCR assay for simultaneous detection of Treponema phagedenis, Treponema pedis, Treponema medium, and 'Treponema vincentii' and evaluation on bovine digital dermatitis biopsies.Veterinary research communications · 2023Article
- An In-House ELISA for Treponema Antibodies in Bulk Milk as Part of a Monitoring Tool for Claw Health in Dairy Herds.Veterinary sciences · 2023Article
- First Molecular Confirmation ofPathogens (Basel, Switzerland) · 2022Article
- Detection ofVeterinary research forum : an international quarterly journal · 2022Article
- Detection and quantification of bacterial species DNA in bovine digital dermatitis lesions in swabs and fine-needle aspiration versus biopsies.Frontiers in veterinary science · 2022Article
- Identification and Quantification of Bovine Digital Dermatitis-Associated Microbiota across Lesion Stages in Feedlot Beef Cattle.mSystems · 2021Article
- Dissecting the molecular diversity and commonality of bovine and human treponemes identifies key survival and adhesion mechanisms.PLoS pathogens · 2021Article
- The dysbiosis of ovine foot microbiome during the development and treatment of contagious ovine digital dermatitis.Animal microbiome · 2021Article
- Article
- First cases of contagious ovine digital dermatitis in Germany.Acta veterinaria Scandinavica · 2020Article
- Survival of bovine digital dermatitis treponemes on hoof knife blades and the effects of various disinfectants.The Veterinary record · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modern pyrosequencing technology allows for a more comprehensive approach than traditional Sanger sequencing for elucidating the etiology of bovine digital dermatitis. We sought to describe the composition and diversity of treponemes in digital dermatitis lesions by using deep sequencing of the V3 and V4 hypervariable regions of the 16S rRNA gene coupled with species-level taxonomic identification. Treponema-specific 16S rRNA gene PCRs and pyrosequencing were performed on biopsy specimens originating from 10 different Catalan dairy herds (n = 36) with digital dermatitis, and this analysis yielded 75,297 sequences. We identified 20 different taxa, including a potentially novel phylotype that displayed 95% sequence identity to members of the Treponema denticola/Treponema pedis-like cluster. Species frequencies and abundances that were determined by pyrosequencing analysis were highly correlated with the results of fluorescent in situ hybridization using phylotype-specific oligonucleotide probes. In a limited number of animals from a single geographic region, we detected most of the Treponema phylotypes that were described in previous investigations of digital dermatitis. Additionally, we identified a number of phylotypes that mapped to oral treponemes of humans and dogs that had not been reported for digital dermatitis lesions. The results presented here support previous observations of a polytreponemal etiology of infections, with Treponema phagedenis-like, Treponema medium/Treponema vincentii-like, and T. denticola/T. pedis-like phylotypes being highly associated with disease. Using this new approach, it has become feasible to study large herds and their surrounding environments, which might provide a basis for a better understanding of the pathogenesis of this disease.
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Registered trials
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