ArticleACS synthetic biology2012
Scalable plasmid transfer using engineered P1-based phagemids.
Article in ACS synthetic biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Cross-order detection of bacteriophage transduction in microbial communities using RNA barcoding.Nature communications · 2026Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Phagemid-based capsid system for CRISPR-Cas13a antimicrobials targeting methicillin-resistant Staphylococcus aureus.Communications biology · 2024Article
- A deoxyviolacein-based transposon insertion vector for pigmented tracer studies.MicrobiologyOpen · 2024Article
- Precision control of ammonium release in Azotobacter vinelandii.Microbial biotechnology · 2024Article
- Interaction of bacteriophage P1 with an epiphyticFrontiers in microbiology · 2024Article
- P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity AgainstACS synthetic biology · 2023Article
- Tail-Engineered Phage P2 Enables Delivery of Antimicrobials into Multiple Gut Pathogens.ACS synthetic biology · 2023Article
- An adenine/thymidine-rich region is integral to RepL-mediated DNA replication.Frontiers in microbiology · 2023Article
- Biological foundations of successful bacteriophage therapy.EMBO molecular medicine · 2022Review
- Inducible directed evolution of complex phenotypes in bacteria.Nucleic acids research · 2022Article
- Genetic Modification ofmSphere · 2021Article
- Diversity of P1 phage-like elements in multidrug resistant Escherichia coli.Scientific reports · 2019Article
- Article
- Two NovelFrontiers in cellular and infection microbiology · 2017Article
- Bacteriophage P1AIMS microbiology · 2017Article
- Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.Nature communications · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Dramatic improvements to computational, robotic, and biological tools have enabled genetic engineers to conduct increasingly sophisticated experiments. Further development of biological tools offers a route to bypass complex or expensive mechanical operations, thereby reducing the time and cost of highly parallelized experiments. Here, we engineer a system based on bacteriophage P1 to transfer DNA from one E. coli cell to another, bypassing the need for intermediate DNA isolation (e.g., minipreps). To initiate plasmid transfer, we refactored a native phage element into a DNA module capable of heterologously inducing phage lysis. After incorporating known cis-acting elements, we identified a novel cis-acting element that further improves transduction efficiency, exemplifying the ability of synthetic systems to offer insight into native ones. The system transfers DNAs up to 25 kilobases, the maximum assayed size, and operates well at microliter volumes, enabling manipulation of most routinely used DNAs. The system's large DNA capacity and physical coupling of phage particles to phagemid DNA suggest applicability to biosynthetic pathway evolution, functional proteomics, and ultimately, diverse molecular biology operations including DNA fabrication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.