ArticleACS synthetic biology2012
Genetic circuit performance under conditions relevant for industrial bioreactors.
Article in ACS synthetic biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed.
- Quantitative Measurement of Synthetic Repression Curves Reveals Design Challenges for Genetic Circuit Engineering under Growth Arrest.ACS synthetic biology · 2026Article
- Living circuit boards built by printing bacterial transistors.Nature chemical biology · 2026Article
- Scalable secondary metabolite production in Streptomyces using a plug-and-play system.Nature biotechnology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- A stationary phase-specific bacterial green light sensor for enhancing metabolite production.Nature communications · 2025Article
- Delaying production with prokaryotic inducible expression systems.Microbial cell factories · 2024Review
- Transfer learning for cross-context prediction of protein expression from 5'UTR sequence.Nucleic acids research · 2024Article
- Bacterial therapies at the interface of synthetic biology and nanomedicine.Nature reviews bioengineering · 2024Article
- Open-endedness in synthetic biology: A route to continual innovation for biological design.Science advances · 2024Review
- Engineered live bacteria as disease detection and diagnosis tools.Journal of biological engineering · 2023Review
- A blueprint for a synthetic genetic feedback optimizer.Nature communications · 2023Article
- Bioindustrial manufacturing readiness levels (BioMRLs) as a shared framework for measuring and communicating the maturity of bioproduct manufacturing processes.Journal of industrial microbiology & biotechnology · 2022Article
- Overcoming Leak Sensitivity in CRISPRi Circuits Using Antisense RNA Sequestration and Regulatory Feedback.ACS synthetic biology · 2022Article
- Synthetic Biological Circuits within an Orthogonal Central Dogma.Trends in biotechnology · 2021Review
- Mathematical modeling of population structure in bioreactors seeded with light-controllable microbial stem cells.Mathematical biosciences and engineering : MBE · 2020Article
- Titrating bacterial growth and chemical biosynthesis for efficient N-acetylglucosamine and N-acetylneuraminic acid bioproduction.Nature communications · 2020Article
- Engineered signal-coupled inducible promoters: measuring the apparent RNA-polymerase resource budget.Nucleic acids research · 2020Article
- Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads.Molecular systems biology · 2020Article
- Tunable genetic devices through simultaneous control of transcription and translation.Nature communications · 2020Article
- Trade-offs between gene expression, growth and phenotypic diversity in microbial populations.Current opinion in biotechnology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Synthetic genetic programs promise to enable novel applications in industrial processes. For such applications, the genetic circuits that compose programs will require fidelity in varying and complex environments. In this work, we report the performance of two synthetic circuits in Escherichia coli under industrially relevant conditions, including the selection of media, strain, and growth rate. We test and compare two transcriptional circuits: an AND and a NOR gate. In E. coli DH10B, the AND gate is inactive in minimal media; activity can be rescued by supplementing the media and transferring the gate into the industrial strain E. coli DS68637 where normal function is observed in minimal media. In contrast, the NOR gate is robust to media composition and functions similarly in both strains. The AND gate is evaluated at three stages of early scale-up: 100 mL shake flask experiments, a 1 mL MTP microreactor, and a 10 L bioreactor. A reference plasmid that constitutively produces a GFP reporter is used to make comparisons of circuit performance across conditions. The AND gate function is quantitatively different at each scale. The output deteriorates late in fermentation after the shift from exponential to constant feed rates, which induces rapid resource depletion and changes in growth rate. In addition, one of the output states of the AND gate failed in the bioreactor, effectively making it only responsive to a single input. Finally, cells carrying the AND gate show considerably less accumulation of biomass. Overall, these results highlight challenges and suggest modified strategies for developing and characterizing genetic circuits that function reliably during fermentation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.