ArticleACS synthetic biology2013
Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites.
Article in ACS synthetic biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed, 223 citations in OpenAlex.
- Evaluation of Antibacterial Properties of Nanosuspension of Secondary Metabolites from Streptomyces chrestomyceticus ADP4.Current microbiology · 2026Article
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- Synthetic Biology Strategies for Activating Cryptic BGCs inACS omega · 2026Review
- Streptomyces venezuelae uses secreted chitinases and a designated ABC transporter to support the competitive saprophytic catabolism of chitin.PLoS biology · 2025Article
- CASCADE-Cas3 enables highly efficient genome engineering in Streptomyces species.Nucleic acids research · 2025Article
- In vivo and in vitro Reconstitution of Biosynthesis of N-Prenylated Phenazines Revealing Diverse Phenazine-Modifying Enzymes.Chembiochem : a European journal of chemical biology · 2025Article
- Stepwise increase of fidaxomicin in an engineered heterologous hostSynthetic and systems biotechnology · 2024Article
- Heterologous expression of lasso peptides with apparent participation in the morphological development in Streptomyces.AMB Express · 2024Article
- Potential ofMetabolites · 2024Review
- Discovery, characterization, and engineering of an advantageous Streptomyces host for heterologous expression of natural product biosynthetic gene clusters.Microbial cell factories · 2024Article
- Combinatorial metabolic engineering of Streptomyces sp. CB03234-S for the enhanced production of anthraquinone-fused enediyne tiancimycins.Microbial cell factories · 2024Article
- Capability of a large bacterial artificial chromosome clone harboring multiple biosynthetic gene clusters for the production of diverse compounds.The Journal of antibiotics · 2024Article
- Genomic Insights and Synthetic Biology Applications of Marine ActinomyceteInternational journal of molecular sciences · 2024Article
- Promising non-model microbial cell factories obtained by genome reduction.Frontiers in bioengineering and biotechnology · 2024Review
- Rationally Improving Doramectin Production in IndustrialBioengineering (Basel, Switzerland) · 2023Article
- Miramides A-D: Identification of Detoxin-like Depsipeptides after Heterologous Expression of a Hybrid NRPS-PKS Gene Cluster fromMicroorganisms · 2022Article
- Discovery of prescopranone, a key intermediate in scopranone biosynthesis.The Journal of antibiotics · 2022Article
- Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production inAntibiotics (Basel, Switzerland) · 2022Article
- Multiplying the heterologous production of spinosad through tandem amplification of its biosynthetic gene cluster in Streptomyces coelicolor.Microbial biotechnology · 2022Article
- Complete Genome Sequence ofMicrobiology resource announcements · 2022Article
46 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
An industrial microorganism, Streptomyces avermitilis, which is a producer of anthelmintic macrocyclic lactones, avermectins, has been constructed as a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis. Twenty of the entire biosynthetic gene clusters for secondary metabolites were successively cloned and introduced into a versatile model host S. avermitilis SUKA17 or 22. Almost all S. avermitilis transformants carrying the entire gene cluster produced metabolites as a result of the expression of biosynthetic gene clusters introduced. A few transformants were unable to produce metabolites, but their production was restored by the expression of biosynthetic genes using an alternative promoter or the expression of a regulatory gene in the gene cluster that controls the expression of biosynthetic genes in the cluster using an alternative promoter. Production of metabolites in some transformants of the versatile host was higher than that of the original producers, and cryptic biosynthetic gene clusters in the original producer were also expressed in a versatile host.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.