ArticleFrontiers in cellular neuroscience2013
Studying subcellular detail in fixed astrocytes: dissociation of morphologically intact glial cells (DIMIGs).
Article in Frontiers in cellular neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Construction and Applicability Scenarios of 3D Neurovascular Unit Models In Vitro.Biomolecules · 2026Review
- Light-based modulation of astrocytic calcium for regulation of organelle dynamics and morphogenesis.The Journal of cell biology · 2026Article
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- Mitochondrial fission controls astrocyte morphogenesis and organization in the cortex.The Journal of cell biology · 2025Article
- Role of Cytoskeletal Elements in Regulation of Synaptic Functions: Implications Toward Alzheimer's Disease and Phytochemicals-Based Interventions.Molecular neurobiology · 2024Review
- Role of spinal astrocytes through the perisynaptic astrocytic process in pathological pain.Molecular brain · 2023Review
- mGluR5 in Astrocytes in the Ventromedial Hypothalamus Regulates Pituitary Adenylate Cyclase-Activating Polypeptide Neurons and Glucose Homeostasis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Article
- A closer look at astrocyte morphology: Development, heterogeneity, and plasticity at astrocyte leaflets.Current opinion in neurobiology · 2022Review
- Mapping microglia and astrocyte activation in vivo using diffusion MRI.Science advances · 2022Article
- Interactions of neuroimmune signaling and glutamate plasticity in addiction.Journal of neuroinflammation · 2021Review
- Glial Patchwork: Oligodendrocyte Progenitor Cells and Astrocytes Blanket the Central Nervous System.Frontiers in cellular neuroscience · 2021Review
- Signatures of the Consolidated Response of Astrocytes to Ischemic Factors In Vitro.International journal of molecular sciences · 2020Article
- ApoE4 Astrocytes Secrete Basement Membranes Rich in Fibronectin and Poor in Laminin Compared to ApoE3 Astrocytes.International journal of molecular sciences · 2020Article
- Quantifying the Local Mechanical Properties of Cells in a Fibrous Three-Dimensional Microenvironment.Biophysical journal · 2019Article
- Effects of Methamphetamine Self-Administration and Extinction on Astrocyte Structure and Function in the Nucleus Accumbens Core.Neuroscience · 2019Article
- Astrocytes enhance the tolerance of rat cortical neurons to glutamate excitotoxicity.Molecular medicine reports · 2019Article
- Pyridazine-derivatives Enhance Structural and Functional Plasticity of Tripartite Synapse Via Activation of Local Translation in Astrocytic Processes.Neuroscience · 2018Article
- Important Shapeshifter: Mechanisms Allowing Astrocytes to Respond to the Changing Nervous System During Development, Injury and Disease.Frontiers in cellular neuroscience · 2018Article
- Correlative STED and Atomic Force Microscopy on Live Astrocytes Reveals Plasticity of Cytoskeletal Structure and Membrane Physical Properties during Polarized Migration.Frontiers in cellular neuroscience · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studying the distribution of astrocytic antigens is particularly hard when they are localized in their fine, peripheral astrocyte processes (PAPs), since these processes often have a diameter comparable to vesicles and small organelles. The most appropriate technique is immunoelectron microscopy, which is, however, a time-consuming procedure. Even in high resolution light microscopy, antigen localization is difficult to detect due to the small dimensions of these processes, and overlay from antigen in surrounding non-glial cells. Yet, PAPs frequently display antigens related to motility and glia-synaptic interaction. Here, we describe the dissociation of morphologically intact glial cells (DIMIGs), permitting unambiguous antigen localization using epifluorescence microscopy. Astrocytes are dissociated from juvenile (p13-15) mouse cortex by applying papain treatment and cytospin centrifugation to attach the cells to a slide. The cells and their complete processes including the PAPs is thus projected in 2D. The entire procedure takes 2.5-3 h. We show by morphometry that the diameter of DIMIGs, including the PAPs is similar to that of astrocytes in situ. In contrast to cell culture, results derived from this procedure allow for direct conclusions relating to (1) the presence of an antigen in cortical astrocytes, (2) subcellular antigen distribution, in particular when localized in the PAPs. The detailed resolution is shown in an exemplary study of the organization of the astrocytic cytoskeleton components actin, ezrin, tubulin, and GFAP. The distribution of connexin 43 in relation to a single astrocyte's process tree is also investigated.
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