ArticleMolecular microbiology2013
Ustilago maydis natural antisense transcript expression alters mRNA stability and pathogenesis.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- A long non-coding RNA,mSphere · 2026Article
- A long non-coding RNA,bioRxiv : the preprint server for biology · 2026Article
- Evaluating Gene Expression in Ustilago maydis Using RT-qPCR.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Identification and Functions of lncRNAs in Fungi.Non-coding RNA · 2025Review
- Characterization of RNA Helicase Genes inInternational journal of molecular sciences · 2025Article
- Annotation and functional prediction of RNA helicases inIMA fungus · 2025Review
- Correlation Between Effector Gene Expression Targeted by lncRNAs in the Oomycete Fish Pathogen, Saprolegnia parasitica.Marine biotechnology (New York, N.Y.) · 2024Article
- The Identification and Comparative Analysis of Non-Coding RNAs in Spores and Mycelia ofJournal of fungi (Basel, Switzerland) · 2023Article
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Discovery of long non-coding RNAs in Aspergillus flavus response to water activity, COScientific reports · 2023Article
- Identification of Long Intergenic Noncoding RNAs inMicrobiology spectrum · 2023Article
- New insights into genome annotation in Podospora anserina through re-exploiting multiple RNA-seq data.BMC genomics · 2022Article
- Genome-wide profiling of long non-coding RNA of the rice blast fungus Magnaporthe oryzae during infection.BMC genomics · 2022Article
- Article
- lncRsp1, a long noncoding RNA, influences Fgsp1 expression and sexual reproduction in Fusarium graminearum.Molecular plant pathology · 2022Article
- The production and secretion of tRNA-derived RNA fragments in the corn smut fungusFrontiers in fungal biology · 2022Article
- An Overview on Identification and Regulatory Mechanisms of Long Non-coding RNAs in Fungi.Frontiers in microbiology · 2021Review
- Genome-Wide Identification and Functional Prediction of Long Non-coding RNAs Involved in the Heat Stress Response inFrontiers in microbiology · 2019Article
- Genome-wide identification of long non-coding RNAs suggests a potential association with effector gene transcription in Phytophthora sojae.Molecular plant pathology · 2018Article
- Transcriptome analysis of smut fungi reveals widespread intergenic transcription and conserved antisense transcript expression.BMC genomics · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ustilago maydis infection of Zea mays leads to the production of thick-walled diploid teliospores that are the dispersal agent for this pathogen. Transcriptome analyses of this model biotrophic basidiomycete fungus identified natural antisense transcripts (NATs) complementary to 247 open reading frames. The U. maydis NAT cDNAs were fully sequenced and annotated. Strand-specific RT-PCR screens confirmed expression and identified NATs preferentially expressed in the teliospore. Targeted screens revealed four U. maydis NATs that are conserved in a related fungus. Expression of NATs in haploid cells, where they are not naturally occurring, resulted in increased steady-state levels of some complementary mRNAs. The expression of one NAT, as-um02151, in haploid cells resulted in a twofold increase in complementary mRNA levels, the formation of sense-antisense double-stranded RNAs, and unchanged Um02151 protein levels. This led to a model for NAT function in the maintenance and expression of stored teliospore mRNAs. In testing this model by deletion of the regulatory region, it was determined that alteration in NAT expression resulted in decreased pathogenesis in both cob and seedling infections. This annotation and functional analysis supports multiple roles for U. maydis NATs in controlling gene expression and influencing pathogenesis.
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