ArticleFEBS open bio2012
Identification of condition-specific reference genes from microarray data for locusts exposed to hypobaric hypoxia.
Article in FEBS open bio, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 11 citations in OpenAlex.
- Identification of Internal Reference Genes in Peripheral Blood Mononuclear Cells of Cattle Populations Adapted to Hot Arid Normoxia and Cold Arid Hypoxia Environments.Frontiers in genetics · 2021Article
- Reference gene selection and validation for mRNA expression analysis by RT-qPCR in murine M1- and M2-polarized macrophage.Molecular biology reports · 2020Article
- Selection of Reference Genes for Normalization of Real-Time PCR Data in Calliptamus italicus (Orthoptera: Acrididae) Under Different Temperature Conditions.Journal of insect science (Online) · 2019Article
- RNA-seq-based selection of reference genes for RT-qPCR analysis of pitaya.FEBS open bio · 2019Article
- Selection of reference genes for qPCR- and ddPCR-based analyses of gene expression in Senescing Barley leaves.PloS one · 2015Article
- Selection of reference genes for gene expression studies related to intramuscular fat deposition in Capra hircus skeletal muscle.PloS one · 2015Article
- Validation of housekeeping genes in the brains of rats submitted to chronic intermittent hypoxia, a sleep apnea model.PloS one · 2014Article
- Article
- Efficient utilization of aerobic metabolism helps Tibetan locusts conquer hypoxia.BMC genomics · 2013Article
- Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia.Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e TisilogiaArticle
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Real-time quantitative polymerase chain reaction (qPCR) is a routine and robust approach for measuring gene expression. The stability of reference genes in qPCR is crucial for the accurate quantification of gene expression. To provide reliable reference genes for studying the transcriptional responses of locust muscles to hypobaric hypoxia, we first examined the gene expression stability of the frequently used housekeeping genes 18S, GAPDH, and β-actin. However, the expression of these three housekeeping genes was influenced by hypobaric hypoxia. Consequently, we identified five novel candidate reference genes from the locust microarray data. The gene expression stability of the five candidates, together with the three classical housekeeping genes, were evaluated using two distinct algorithms implemented in geNorm and NormFinder. GeNorm identified Ach (acetyl-CoA hydrolase) and Pgp (phosphoglycolate phosphatase-like) as the most stable genes and NormFinder further distinguished Ach as the most stable one. The validity of Ach as a reference gene was confirmed through comparison with 18S. This study exemplifies the necessity of validating reference genes before their application and the feasibility of identifying condition-specific reference genes from large-scale gene expression data.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.