ArticleFEBS open bio2012
Evolution of an inhibitory RNA aptamer against T7 RNA polymerase.
Article in FEBS open bio, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Synthetic dynamic transcription frameworks and their applications.Nature chemistry · 2026Review
- Plasmids for Controlled and Tunable High-Level Expression in E. coli.Applied and environmental microbiology · 2022Article
- Autoinhibited transient, gated, and cascaded dynamic transcription of RNAs.Science advances · 2022Article
- Building an RNA-Based Toggle Switch Using Inhibitory RNA Aptamers.ACS synthetic biology · 2022Article
- Current Advances in the Development of Diagnostic Tests based on Aptamers in Parasitology: A Systematic Review.Pharmaceutics · 2020Review
- Switching the activity of Taq polymerase using clamp-like triplex aptamer structure.Nucleic acids research · 2020Article
- Promoter RNA sequencing (PRSeq) for the massive and quantitative promoter analysis in vitro.Scientific reports · 2019Article
- Altered stoichiometry of an evolved RNA aptamer.RNA (New York, N.Y.) · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aptamers are promising gene components that can be used for the construction of synthetic gene circuits. In this study, we isolated an RNA aptamer that specifically inhibits transcription of T7 RNA polymerase (RNAP). The 38-nucleotide aptamer, which was a shortened variant of an initial SELEX isolate, showed moderate inhibitory activity. By stepwise doped-SELEX, we isolated evolved variants with strong inhibitory activity. A 29-nucleotide variant of a doped-SELEX isolate showed 50% inhibitory concentration at 11 nM under typical in vitro transcription conditions. Pull-down experiments revealed that the aptamer inhibited the association of T7 RNAP with T7 promoter DNA.
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Registered trials
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