ArticleProceedings of the National Academy of Sciences of the United States of America2013
The posttranslational modification cascade to the thiopeptide berninamycin generates linear forms and altered macrocyclic scaffolds.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Exploring the biosynthetic potential of Korean Actinobacteria for antibacterial metabolite discovery.Journal of microbiology (Seoul, Korea) · 2025Article
- Discovery, characterization, and engineering of an advantageous Streptomyces host for heterologous expression of natural product biosynthetic gene clusters.Microbial cell factories · 2024Article
- Core-dependent post-translational modifications guide the biosynthesis of a new class of hypermodified peptides.Nature communications · 2023Article
- Cytochromes P450 Associated with the Biosyntheses of Ribosomally Synthesized and Post-translationally Modified Peptides.ACS bio & med chem Au · 2023Review
- An antimicrobial thiopeptide producing novel actinomycetesFEMS microbes · 2023Article
- Ψ-Footprinting approach for the identification of protein synthesis inhibitor producers.NAR genomics and bioinformatics · 2022Article
- Antibiofilm activity of Cutibacterium acnes cell-free conditioned media against Staphylococcus spp.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Streptomonospora litoralis sp. nov., a halophilic thiopeptides producer isolated from sand collected at Cuxhaven beach.Antonie van Leeuwenhoek · 2021Article
- Thirtieth Anniversary of the Discovery of Laxaphycins. Intriguing Peptides Keeping a Part of Their Mystery.Marine drugs · 2021Review
- New developments in RiPP discovery, enzymology and engineering.Natural product reports · 2021Review
- AScience translational medicine · 2020Article
- Litoralimycins A and B, New Cytotoxic Thiopeptides fromMarine drugs · 2020Article
- Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering.Microbial biotechnology · 2019Review
- Heterologous Production of Microbial Ribosomally Synthesized and Post-translationally Modified Peptides.Frontiers in microbiology · 2018Review
- Genome Mining ofFrontiers in microbiology · 2018Article
- Elucidating and engineering thiopeptide biosynthesis.World journal of microbiology & biotechnology · 2017Review
- YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function.Chemical reviews · 2017Review
- Mutagenesis of NosM Leader Peptide Reveals Important Elements in Nosiheptide Biosynthesis.Applied and environmental microbiology · 2017Article
- Cloning and Heterologous Expression of a Large-sized Natural Product Biosynthetic Gene Cluster inFrontiers in microbiology · 2017Review
- An α/β-hydrolase fold protein in the biosynthesis of thiostrepton exhibits a dual activity for endopeptidyl hydrolysis and epoxide ring opening/macrocyclization.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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5 authors.
Funding
Abstract
Berninamycin is a member of the pyridine-containing thiopeptide class of antibiotics that undergoes massive posttranslational modifications from ribosomally generated preproteins. Berninamycin has a 2-oxazolyl-3-thiazolyl-pyridine core embedded in a 35-atom macrocycle rather than typical trithiazolylpyridine cores embedded in 26-atom and 29-atom peptide macrocycles. We describe the cloning of an 11-gene berninamycin cluster from Streptomyces bernensis UC 5144, its heterologous expression in Streptomyces lividans TK24 and Streptomyces venezuelae ATCC 10712, and detection of variant and incompletely processed scaffolds. Posttranslational maturation in S. lividans of both the wild-type berninamycin prepeptide (BerA) and also a T3A mutant generates macrocyclic compounds as well as linear variants, which have failed to form the pyridine and the macrocycle. Expression of the gene cluster in S. venezuelae generates a variant of the 35-atom skeleton of berninamycin, containing a methyloxazoline in the place of a methyloxazole within the macrocyclic framework.
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