ArticleProceedings of the National Academy of Sciences of the United States of America2013
Visualizing cellular interactions with a generalized proximity reporter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection.Nature biomedical engineering · 2025Article
- DNA-Directed Activation of Photocatalytic Labeling at Cell-Cell Contact Sites.ACS chemical biology · 2024Article
- Visualizing cell-cell communication using synthetic notch activated MRI.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Hydrolysis-Resistant Ester-Based Linkers for Development of Activity-Based NIR Bioluminescence Probes.Journal of the American Chemical Society · 2023Article
- Caged Cumate Enables Proximity-Dependent Control Over Gene Expression.Chembiochem : a European journal of chemical biology · 2021Article
- A Chemical Approach for Programmable Protein Outputs Based on Engineered Cell Interactions.ACS chemical biology · 2021Article
- Interrogating Cellular Communication in Cancer with Genetically Encoded Imaging Reporters.Radiology. Imaging cancer · 2020Review
- Bioluminescence imaging in mice with synthetic luciferin analogues.Methods in enzymology · 2020Article
- Caged luciferins for bioluminescent activity-based sensing.Current opinion in biotechnology · 2019Review
- Development of a Split Esterase for Protein-Protein Interaction-Dependent Small-Molecule Activation.ACS central science · 2019Article
- Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging.Chembiochem : a European journal of chemical biology · 2018Article
- In vivo bioluminescence imaging of labile iron accumulation in a murine model ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Molecular Imaging in Synthetic Biology, and Synthetic Biology in Molecular Imaging.Molecular imaging and biology · 2017Review
- In vivo bioluminescence imaging reveals copper deficiency in a murine model of nonalcoholic fatty liver disease.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Expanding Toolbox of In Vivo Bioluminescent Imaging.Frontiers in oncology · 2016Review
- Rapid and scalable assembly of firefly luciferase substrates.Organic & biomolecular chemistry · 2015Article
- Article
- Beyond D-luciferin: expanding the scope of bioluminescence imaging in vivo.Current opinion in chemical biology · 2014Review
- Clonal analyses in the anterior pre-placodal region: implications for the early lineage bias of placodal progenitors.The International journal of developmental biology · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Interactions among neighboring cells underpin many physiological processes ranging from early development to immune responses. When these interactions do not function properly, numerous pathologies, including infection and cancer, can result. Molecular imaging technologies, especially optical imaging, are uniquely suited to illuminate complex cellular interactions within the context of living tissues in the body. However, no tools yet exist that allow the detection of microscopic events, such as two cells coming into close proximity, on a global, whole-animal scale. We report here a broadly applicable, longitudinal strategy for probing interactions among cells in living subjects. This approach relies on the generation of bioluminescent light when two distinct cell populations come into close proximity, with the intensity of the optical signal correlating with relative cellular location. We demonstrate the ability of this reporter strategy to gauge cell-cell proximity in culture models in vitro and then evaluate this approach for imaging tumor-immune cell interactions using a murine breast cancer model. In these studies, our imaging strategy enabled the facile visualization of features that are otherwise difficult to observe with conventional imaging techniques, including detection of micrometastatic lesions and potential sites of tumor immunosurveillance. This proximity reporter will facilitate probing of numerous types of cell-cell interactions and will stimulate the development of similar techniques to detect rare events and pathological processes in live animals.
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Registered trials
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