ArticleEnvironmental health perspectives2013
Interlaboratory evaluation of in vitro cytotoxicity and inflammatory responses to engineered nanomaterials: the NIEHS Nano GO Consortium.
Article in Environmental health perspectives, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
103 citing papers in PubMed, 204 citations in OpenAlex.
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- The Assessment of Bioactivity and Biological Responsiveness in Bioactive Glasses and Ceramics: A Review of Available Techniques.Materials (Basel, Switzerland) · 2025Review
- The role of NLRP3 inflammasome activation in proinflammatory and cytotoxic effects of metal nanoparticles.Archives of toxicology · 2025Review
- Toward Standardization of a Lung New Approach Model for Toxicity Testing of Nanomaterials.Nanomaterials (Basel, Switzerland) · 2024Article
- In vitro inflammation and toxicity assessment of pre- and post-incinerated organomodified nanoclays to macrophages using high-throughput screening approaches.Particle and fibre toxicology · 2024Article
- Interaction of TiOEnvironmental science. Nano · 2023Article
- U. S. federal perspective on critical research issues in nanoEHS.Environmental science. Nano · 2023Article
- Interlaboratory comparison of an intestinal triple culture to confirm transferability and reproducibility.In vitro models · 2023Article
- MMP-3-mediated cleavage of OPN is involved in copper oxide nanoparticle-induced activation of fibroblasts.Particle and fibre toxicology · 2023Article
- Contribution of toxicological pathology to occupational health: lung carcinogenicity of fibrous and particulate substances in rats.Journal of toxicologic pathology · 2023Review
- The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design.Nanomaterials (Basel, Switzerland) · 2023Review
- Exploring the Antimicrobial and Pharmacological Potential of NF22 as a Potent Inhibitor ofPharmaceutics · 2022Article
- Influence of Impurities from Manufacturing Process on the Toxicity Profile of Boron Nitride Nanotubes.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- Potential Artifacts and Control Experiments in Toxicity Tests of Nanoplastic and Microplastic Particles.Environmental science & technology · 2022Review
- Interlaboratory evaluation of a digital holographic microscopy-based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriers.Drug delivery and translational research · 2022Article
- Standardization of an in vitro assay matrix to assess cytotoxicity of organic nanocarriers: a pilot interlaboratory comparison.Drug delivery and translational research · 2022Article
- Review
- How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test.Nanomaterials (Basel, Switzerland) · 2022Article
43 more citing papers are in PubMed but not listed here.
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Authors and funding
24 authors at 10 institutions in 1 country.
Funding
Abstract
backgroundDifferences in interlaboratory research protocols contribute to the conflicting data in the literature regarding engineered nanomaterial (ENM) bioactivity.
objectivesGrantees of a National Institute of Health Sciences (NIEHS)-funded consortium program performed two phases of in vitro testing with selected ENMs in an effort to identify and minimize sources of variability.
methodsConsortium program participants (CPPs) conducted ENM bioactivity evaluations on zinc oxide (ZnO), three forms of titanium dioxide (TiO2), and three forms of multiwalled carbon nanotubes (MWCNTs). In addition, CPPs performed bioassays using three mammalian cell lines (BEAS-2B, RLE-6TN, and THP-1) selected in order to cover two different species (rat and human), two different lung epithelial cells (alveolar type II and bronchial epithelial cells), and two different cell types (epithelial cells and macrophages). CPPs also measured cytotoxicity in all cell types while measuring inflammasome activation [interleukin-1β (IL-1β) release] using only THP-1 cells.
resultsThe overall in vitro toxicity profiles of ENM were as follows: ZnO was cytotoxic to all cell types at ≥ 50 μg/mL, but did not induce IL-1β. TiO2 was not cytotoxic except for the nanobelt form, which was cytotoxic and induced significant IL-1β production in THP-1 cells. MWCNTs did not produce cytotoxicity, but stimulated lower levels of IL-1β production in THP-1 cells, with the original MWCNT producing the most IL-1β.
conclusionsThe results provide justification for the inclusion of mechanism-linked bioactivity assays along with traditional cytotoxicity assays for in vitro screening. In addition, the results suggest that conducting studies with multiple relevant cell types to avoid false-negative outcomes is critical for accurate evaluation of ENM bioactivity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.