ArticleInfection and immunity2013
The Dot/Icm effector SdhA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- The cGAS-STING signaling pathway in the regulation of pulmonary infections: a systematic review.Frontiers in cellular and infection microbiology · 2025Pooled it
- The common HAQ STING variant impairs cGAS-dependent antibacterial responses and is associated with susceptibility to Legionnaires' disease in humans.PLoS pathogens · 2018Trial
- Review
- Evaluation of Amlodipine and Imipramine Efficacy to TreatAntibiotics (Basel, Switzerland) · 2025Article
- Pattern Recognition by NOD-Like Receptors.Advances in experimental medicine and biology · 2025Review
- Review
- Investigating Secretion Systems and Effectors on Galleria mellonella.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Concept about the Virulence Factor ofMicroorganisms · 2022Review
- Characterization of a Novel Regulator of Biofilm Formation in the PathogenBiomolecules · 2022Article
- Knowledge to Predict Pathogens:Microorganisms · 2022Review
- Inhibition of the master regulator of Listeria monocytogenes virulence enables bacterial clearance from spacious replication vacuoles in infected macrophages.PLoS pathogens · 2022Article
- Virulence effector SidJ evolution inPeerJ · 2021Article
- Review
- Mitigation of Expression of Virulence Genes inPathogens (Basel, Switzerland) · 2020Article
- Paradigms of Protist/Bacteria Symbioses Affecting Human Health:Frontiers in microbiology · 2020Review
- Differences in Virulence BetweenFrontiers in cellular and infection microbiology · 2018Article
- The Influence ofFrontiers in microbiology · 2018Article
- The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence.Gut microbes · 2017Article
- The SCID Mouse Model for Identifying Virulence Determinants inFrontiers in cellular and infection microbiology · 2017Article
- The Toolbox for Uncovering the Functions ofFrontiers in cellular and infection microbiology · 2017Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Legionella pneumophila is an intracellular bacterium that resides within amoebae and macrophages in a specialized compartment termed the Legionella-containing vacuole (LCV). As well as providing an intracellular niche for replication, the LCV helps to prevent the release of bacterial components into the cytoplasm. Recognition of these components as danger signals by the host activates immune responses leading to clearance of the bacterium. Here, we examined the role of two important virulence factors of L. pneumophila, the potent danger signal flagellin and the translocated Dot/Icm type IVB secretion system effector SdhA, which is crucial to maintain LCV integrity, in the Galleria mellonella infection model. We demonstrate that flagellin expression does not contribute to virulence, replication, or induction of clearance mechanisms. Conversely, SdhA expression is important for virulence. We found that in the absence of SdhA, the LCV in hemocytes showed signs of instability and leakage. Furthermore, in contrast to wild-type L. pneumophila, a ΔsdhA mutant caused a transient depletion of hemocytes and reduced mortality. Analysis of the ΔsdhA mutant in the A/J mouse model also showed a significant replication defect. Together, our data underline the crucial importance of SdhA in infection across different model organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.