ArticleJournal of the American Chemical Society2013
Real-time detection of isothermal amplification reactions with thermostable catalytic hairpin assembly.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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Who cites it
57 citing papers in PubMed.
- Translating Nature's Design Rules: How Catalysis and Life Science Guide Molecular Catassembly.JACS Au · 2026Review
- Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis.Scientific reports · 2025Article
- Review
- Rapid Amplification and Detection of Single-Stranded Nucleic Acids for Point-of-Care Diagnosis.Small methods · 2025Review
- Robust Sequence Design Space for the Isothermal Exponential Amplification of Short Oligonucleotides.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- A gold nanoparticle conjugated single-legged DNA walker driven by catalytic hairpin assembly biosensor to detect a prokaryotic pathogen.Scientific reports · 2024Article
- Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus.Journal of biosciences · 2024Article
- Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection.Talanta · 2023Review
- Cascaded, Feedback-Driven, and Spatially Localized Emergence of Constitutional Dynamic Networks Driven by Enzyme-Free Catalytic DNA Circuits.Journal of the American Chemical Society · 2023Article
- Programmable mismatch-fueled high-efficiency DNA signal amplifier.Chemical science · 2022Article
- Facile, Rapid, and Low-Cost Detection for Influenza Viruses and Respiratory Syncytial Virus Based on a Catalytic DNA Assembly Circuit.ACS omega · 2022Article
- Chemiluminescent screening of specific hybridoma cells via a proximity-rolling circle activated enzymatic switch.Communications biology · 2022Article
- Programming cell-free biosensors with DNA strand displacement circuits.Nature chemical biology · 2022Article
- Dual-color blending based visual LAMP for food allergen detection: A strategy with enlarged color variation range and contrast.Food chemistry: X · 2022Article
- Programmable High-Speed and Hyper-Efficiency DNA Signal Magnifier.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Developing predictive hybridization models for phosphorothioate oligonucleotides using high-resolution melting.PloS one · 2022Article
- Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors.Molecules (Basel, Switzerland) · 2021Review
- Catalytic Amplification of Electrochemical Signal in Homogeneous Solution Using an Entropy-driven DNA Circuit.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Nucleic Acids Analysis.Science China. Chemistry · 2021Review
- The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond.Trends in analytical chemistry : TRAC · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Catalytic hairpin assembly (CHA) is an enzyme-free amplification method that has previously proven useful in amplifying and transducing signals at the terminus of nucleic acid amplification reactions. Here, for the first time, we engineered CHA to be thermostable from 37 to 60 °C and in consequence have generalized its application to the real-time detection of isothermal amplification reactions. CHA circuits were designed and optimized for both high- and low-temperature rolling circle amplification (RCA) and strand displacement amplification (SDA). The resulting circuits not only increased the specificity of detection but also improved the sensitivity by as much as 25- to 10000-fold over comparable real-time detection methods. These methods have been condensed into a set of general rules for the design of thermostable CHA circuits with high signals and low noise.
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Registered trials
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