ArticleBiomacromolecules2013
Nephila clavipes Flagelliform silk-like GGX motifs contribute to extensibility and spacer motifs contribute to strength in synthetic spider silk fibers.
Article in Biomacromolecules, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 73 citations in OpenAlex.
- A newly evolved small secretory peptide enhances mechanical properties of spider silk.Nature communications · 2025Article
- Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications.Biomimetics (Basel, Switzerland) · 2024Review
- Efficient Biosynthetic Fabrication of Spidroins with High Spinning Performance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- Influence of Spider Silk Protein Structure on Mechanical and Biological Properties for Energetic Material Detection.Molecules (Basel, Switzerland) · 2024Review
- Protein-Based Biological Materials: Molecular Design and Artificial Production.Chemical reviews · 2023Review
- Spider Silk-Inspired Artificial Fibers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Customized Flagelliform Spidroins Form Spider Silk-like Fibers at pH 8.0 with Outstanding Tensile Strength.ACS biomaterials science & engineering · 2022Article
- Recombinant Spider Silk: Promises and Bottlenecks.Frontiers in bioengineering and biotechnology · 2022Review
- Molecular Dynamics of Synthetic Flagelliform Silk Fiber Assembly.Macromolecular materials and engineering · 2021Article
- Expanding Canonical Spider Silk Properties through a DNA Combinatorial Approach.Materials (Basel, Switzerland) · 2020Article
- Engineering Silk Materials: From Natural Spinning to Artificial Processing.Applied physics reviews · 2020Article
- Toward Spider Glue: Long Read Scaffolding for Extreme Length and Repetitious Silk Family Genes AgSp1 and AgSp2 with Insights into Functional Adaptation.G3 (Bethesda, Md.) · 2019Article
- Spider silk proteome provides insight into the structural characterization of Nephila clavipes flagelliform spidroin.Scientific reports · 2018Article
- Spider dragline silk composite films doped with linear and telechelic polyalanine: Effect of polyalanine on the structure and mechanical properties.Scientific reports · 2018Article
- The Nephila clavipes genome highlights the diversity of spider silk genes and their complex expression.Nature genetics · 2017Article
- The correlation between the length of repetitive domain and mechanical properties of the recombinant flagelliform spidroin.Biology open · 2017Article
- Biomimetic spinning of artificial spider silk from a chimeric minispidroin.Nature chemical biology · 2017Article
- Importance of Heat and Pressure for Solubilization of Recombinant Spider Silk Proteins in Aqueous Solution.International journal of molecular sciences · 2016Article
- Crystal Structure of the Nephila clavipes Major Ampullate Spidroin 1A N-terminal Domain Reveals Plasticity at the Dimer Interface.The Journal of biological chemistry · 2016Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Flagelliform spider silk is the most extensible silk fiber produced by orb weaver spiders, though not as strong as the dragline silk of the spider. The motifs found in the core of the Nephila clavipes flagelliform Flag protein are GGX, spacer, and GPGGX. Flag does not contain the polyalanine motif known to provide the strength of dragline silk. To investigate the source of flagelliform fiber strength, four recombinant proteins were produced containing variations of the three core motifs of the Nephila clavipes flagelliform Flag protein that produces this type of fiber. The as-spun fibers were processed in 80% aqueous isopropanol using a standardized process for all four fiber types, which produced improved mechanical properties. Mechanical testing of the recombinant proteins determined that the GGX motif contributes extensibility and the spacer motif contributes strength to the recombinant fibers. Recombinant protein fibers containing the spacer motif were stronger than the proteins constructed without the spacer that contained only the GGX motif or the combination of the GGX and GPGGX motifs. The mechanical and structural X-ray diffraction analysis of the recombinant fibers provide data that suggests a functional role of the spacer motif that produces tensile strength, though the spacer motif is not clearly defined structurally. These results indicate that the spacer is likely a primary contributor of strength, with the GGX motif supplying mobility to the protein network of native N. clavipes flagelliform silk fibers.
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