ArticleAsian Pacific journal of tropical biomedicine2013
Giemsa and Grocott in the recognition of Histoplasma capsulatum in blood smears.
Article in Asian Pacific journal of tropical biomedicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Mucosal Leishmaniasis of the lip: Report of an Exuberant case in a Young man.Head and neck pathology · 2023Article
- Epididymo-orchitis caused byMedical mycology case reports · 2022Article
- Case report: Disseminated histoplasmosis in a renal transplant recipient from a non-endemic region.Frontiers in pediatrics · 2022Article
- Pulmonary Histoplasmosis Mimicking Metastatic Lung Cancer: A Case Report.Diagnostics (Basel, Switzerland) · 2021Article
- Subacute histoplasmosis with focal involvement of the epiglottis: importance of differential diagnosis.Case reports in otolaryngology · 2014Article
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Abstract
objectiveTo facilitate the recognition of intracellular yeasts of Histoplasma capsulatum and differentiate it from Leishmania amastigotes and other parasites, using the combination of Giemsa and a rapid modification of Grocott stains to peripheral blood smears in a hematological study.
methodsThe combination of both stains was applied consecutively (first Grocott and then Giemsa) to previously fixed peripheral blood smears. Microscopy was performed with 400× and 1 000×, the latter using immersion oil.
resultsThe yeasts of Histoplasma capsulatum were observed into the cytoplasm of leukocytes as brownish oval elements, with 3-4 µm in diameter.
conclusionsThe combination of both techniques is a simple and fast method to facilitate recognition of intracellular yeasts and it is different from intracellular parasitic elements. Moreover, it allows distinguishing the cell elements that are in the microscopic preparations. It may be very helpful in those cases in which the presumptive diagnosis of histoplasmosis has not been established yet and where other more sophisticated methods are not available.
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