ArticlePloS one2013
Characterization of a novel metal-dependent D-psicose 3-epimerase from Clostridium scindens 35704.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 94 citations in OpenAlex.
- Establishment of the Biotransformation of D-Allulose and D-Allose Systems in Full-Red Jujube Monosaccharides.Plants (Basel, Switzerland) · 2023Article
- Produce D-allulose from non-food biomass by integrating corn stalk hydrolysis with whole-cell catalysis.Frontiers in bioengineering and biotechnology · 2023Article
- Optimization of Ultrahigh-Throughput Screening Assay for Protein Engineering of d-Allulose 3-Epimerase.Biomolecules · 2022Article
- Immobilization of D-allulose 3-epimerase into magnetic metal-organic framework nanoparticles for efficient biocatalysis.World journal of microbiology & biotechnology · 2022Article
- Efficient D-allulose synthesis under acidic conditions by auto-inducing expression of the tandem D-allulose 3-epimerase genes in Bacillus subtilis.Microbial cell factories · 2022Article
- Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2022Article
- A Possible Mechanism of Graphene Oxide to Enhance Thermostability of D-Psicose 3-Epimerase Revealed by Molecular Dynamics Simulations.International journal of molecular sciences · 2021Article
- Enhanced Thermostability of D-Psicose 3-Epimerase fromInternational journal of molecular sciences · 2021Article
- Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes.Foods (Basel, Switzerland) · 2021Review
- Crystal structure of a novel homodimeric l-ribulose 3-epimerase from Methylomonus sp.FEBS open bio · 2021Article
- Characterization of a Recombinant D-Allulose 3-epimerase from Thermoclostridium caenicola with Potential Application in D-Allulose Production.Molecular biotechnology · 2021Article
- Rare Sugars: Recent Advances and Their Potential Role in Sustainable Crop Protection.Molecules (Basel, Switzerland) · 2021Review
- D-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat-stability and noteworthy potential of D-fructose epimerization.Microbial cell factories · 2021Article
- Article
- A Novel d-Allulose 3-Epimerase Gene from the Metagenome of a Thermal Aquatic Habitat and d-Allulose Production by Bacillus subtilis Whole-Cell Catalysis.Applied and environmental microbiology · 2020Article
- Review onFrontiers in bioengineering and biotechnology · 2020Review
- Biocatalytic Synthesis of D-Allulose Using Novel D-Tagatose 3-Epimerase FromFrontiers in chemistry · 2020Article
- Galactose to tagatose isomerization at moderate temperatures with high conversion and productivity.Nature communications · 2019Article
- High Conversion of D-Fructose into D-Allulose by Enzymes Coupling with an ATP Regeneration System.Molecular biotechnology · 2019Article
- Redesign of a novel D-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of D-allulose.Microbial cell factories · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The noncharacterized protein CLOSCI_02528 from Clostridium scindens ATCC 35704 was characterized as D-psicose 3-epimerase. The enzyme showed maximum activity at pH 7.5 and 60°C. The half-life of the enzyme at 50°C was 108 min, suggesting the enzyme was relatively thermostable. It was strictly metal-dependent and required Mn²⁺ as optimum cofactor for activity. In addition, Mn²⁺ improved the structural stability during both heat- and urea-induced unfolding. Using circular dichroism measurements, the apparent melting temperature (T m) and the urea midtransition concentration (C m) of metal-free enzyme were 64.4°C and 2.68 M. By comparison, the Mn²⁺-bound enzyme showed higher T m and C m with 67.3°C and 5.09 M. The Michaelis-Menten constant (K m), turnover number (k cat), and catalytic efficiency (k cat/K m) values for substrate D-psicose were estimated to be 28.3 mM, 1826.8 s⁻¹, and 64.5 mM⁻¹ s⁻¹, respectively. The enzyme could effectively produce D-psicose from D-fructose with the turnover ratio of 28%.
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