ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2013
Next-generation whole genome sequencing identifies the direction of norovirus transmission in linked patients.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 86 citations in OpenAlex.
- Unraveling the Transmission Dynamics of a Novel Norovirus GII.17[P17] Lineage During Two Consecutive Outbreaks in a Spanish Hospital.Journal of medical virology · 2026Article
- Identifying intra-hospital Norovirus GII transmission using whole-genome sequencing.Genome medicine · 2026Article
- Current infectious disease management challenges in inborn errors of immunity.Annals of clinical microbiology and antimicrobials · 2025Review
- A general approach to identify low-frequency variants within influenza samples collected during routine surveillance.Microbial genomics · 2022Article
- Detection and Sequencing of Multiple Human Norovirus Genotypes from Imported Frozen Raspberries Linked to Outbreaks in the Province of Quebec, Canada, in 2017.Food and environmental virology · 2022Article
- Prolonged norovirus infections correlate to quasispecies evolution resulting in structural changes of surface-exposed epitopes.iScience · 2021Article
- Genome-wide analyses of human noroviruses provide insights on evolutionary dynamics and evidence of coexisting viral populations evolving under recombination constraints.PLoS pathogens · 2021Article
- Deciphering an Adenovirus F41 Outbreak in Pediatric Hematopoietic Stem Cell Transplant Recipients by Whole-Genome Sequencing.Journal of clinical microbiology · 2021Article
- Complete genome characterization of human noroviruses allows comparison of minor alleles during acute and chronic infections.Access microbiology · 2021Article
- Strategy and Performance Evaluation of Low-Frequency Variant Calling for SARS-CoV-2 Using Targeted Deep Illumina Sequencing.Frontiers in microbiology · 2021Article
- High Throughput Sequencing for the Detection and Characterization of RNA Viruses.Frontiers in microbiology · 2021Review
- Review
- Foodborne viral outbreaks associated with frozen produce.Epidemiology and infection · 2019Review
- Prospective, Real-time Metagenomic Sequencing During Norovirus Outbreak Reveals Discrete Transmission Clusters.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Genomic analysis of respiratory syncytial virus infections in households and utility in inferring who infects the infant.Scientific reports · 2019Article
- Emergence of norovirus strains: A tale of two genes.Virus evolution · 2019Review
- Norovirus Transmission Dynamics in a Pediatric Hospital Using Full Genome Sequences.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Genetic characterization of norovirus GII.4 variants circulating in Canada using a metagenomic technique.BMC infectious diseases · 2018Article
- Comparative phylogenetic analyses of recombinant noroviruses based on different protein-encoding regions show the recombination-associated evolution pattern.Scientific reports · 2017Article
- A universal primer-independent next-generation sequencing approach for investigations of norovirus outbreaks and novel variants.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
Abstract
backgroundNoroviruses are a highly transmissible and major cause of nosocomial gastroenteritis resulting in bed and hospital-ward closures. Where hospital outbreaks are suspected, it is important to determine the routes of spread so that appropriate infection-control procedures can be implemented. To investigate a cluster of norovirus cases occurring in children undergoing bone marrow transplant, we undertook norovirus genome sequencing by next-generation methods. Detailed comparison of sequence data from 2 linked cases enabled us to identify the likely direction of spread.
methodsNorovirus complementary DNA was amplified by overlapping polymerase chain reaction (PCR) from 13 stool samples from 5 diagnostic real-time PCR-positive patients. The amplicons were sequenced by Roche 454, the genomes assembled by de novo assembly, and the data analyzed phylogenetically.
resultsPhylogenetic analysis indicated that patients were infected by viruses similar to 4 distinct GII.4 subtypes and 2 patients were linked by the same virus. Of the 14 sites at which there were differences between the consensus sequences of the 2 linked viral genomes, 9 had minor variants present within one or the other patient. Further analysis confirmed that minor variants at all 9 sites in patient B w ere present as the consensus sequence in patient A.
conclusionsPhylogenetic analysis excluded a common source of infection in this apparent outbreak. Two of 3 patients on the same ward had closely related viruses, raising the possibility of cross-infection despite protective isolation. Analysis of deep sequencing data enabled us to establish the likely direction of nosocomial transmission.
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