ArticleJournal of bacteriology2013
Eep confers lysozyme resistance to enterococcus faecalis via the activation of the extracytoplasmic function sigma factor SigV.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 65 citations in OpenAlex.
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- Characterization of natural product inhibitors of quorum sensing inbioRxiv : the preprint server for biology · 2024Article
- An Overview of the Factors Involved in Biofilm Production by theInternational journal of molecular sciences · 2023Review
- The role of site-2-proteases in bacteria: a review on physiology, virulence, and therapeutic potential.microLife · 2023Review
- Immune-mediated competition benefits protective microbes over pathogens in a novel host species.Heredity · 2022Article
- Disruption of theJournal of bacteriology · 2022Article
- Microbubbles Stabilized by Protein Shell: From Pioneering Ultrasound Contrast Agents to Advanced Theranostic Systems.Pharmaceutics · 2022Review
- The Cationic Antimicrobial Peptide Activity of Lysozyme Reduces ViableAntimicrobial agents and chemotherapy · 2022Article
- Activation of the Extracytoplasmic Function σ Factor σmSphere · 2022Article
- Activation of the extracytoplasmic function σ factor σCurrent opinion in microbiology · 2022Review
- Role of Antimicrobial Drug in the Development of Potential Therapeutics.Evidence-based complementary and alternative medicine : eCAM · 2022Review
- Features and Colonization Strategies ofFrontiers in microbiology · 2022Article
- SigV Mediates Lysozyme Resistance in Enterococcus faecalis via RsiV and PgdA.Journal of bacteriology · 2021Article
- Article
- Exploring the Therapeutic Potenital of the Leaderless Enterocins K1 and EJ97 in the Treatment of Vancomycin-Resistant Enterococcal Infection.Frontiers in microbiology · 2021Article
- Recent Insights Into the Prognostic and Therapeutic Applications of Lysozymes.Frontiers in pharmacology · 2021Review
- Intrinsic resistance of Enterococcus faecalis strains to ΦEf11 phage endolysin is associated with the presence of ΦEf11 prophage.Archives of virology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Enterococcus faecalis is a commensal bacterium found in the gastrointestinal tract of most mammals, including humans, and is one of the leading causes of nosocomial infections. One of the hallmarks of E. faecalis pathogenesis is its unusual ability to tolerate high concentrations of lysozyme, which is an important innate immune component of the host. Previous studies have shown that the presence of lysozyme leads to the activation of SigV, an extracytoplasmic function (ECF) sigma factor in E. faecalis, and that the deletion of sigV increases the susceptibility of the bacterium toward lysozyme. Here, we describe the contribution of Eep, a membrane-bound zinc metalloprotease, to the activation of SigV under lysozyme stress by its effects on the stability of the anti-sigma factor RsiV. We demonstrate that the Δeep mutant phenocopies the ΔsigV mutant in lysozyme, heat, ethanol, and acid stress susceptibility. We also show, using an immunoblot analysis, that in an eep deletion mutant, the anti-sigma factor RsiV is only partially degraded after lysozyme exposure, suggesting that RsiV is processed by unknown protease(s) prior to the action of Eep. An additional observation is that the deletion of rsiV, which results in constitutive SigV expression, leads to chaining of cells, suggesting that SigV might be involved in regulating cell wall-modifying enzymes important in cell wall turnover. We also demonstrate that, in the absence of eep or sigV, enterococci bind significantly more lysozyme, providing a plausible explanation for the increased sensitivity of these mutants toward lysozyme.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.